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Gibco™

MEM, autoclavable, no glutamine, powder

Catalog number: 11700077
Gibco™

MEM, autoclavable, no glutamine, powder

Catalog number: 11700077
Catalog Number
11700077
also known as 11700-077
Unit Size
10 L
Price (USD)
Price: 54.25
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
11700077
also known as 11700-077
10 L
Price: 54.25
Your price:
-
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Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:
WithWithout
• Phenol Red• L-glutamine
• Earle's salts• HEPES
 • Sodium Bicarbonate


The complete formulation is available.

Gibco MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. To allow autoclaving this medium, choline chloride is substituted with choline bitartrate, phenol red is reduced, and succinic acid is added to prevent precipitation at low pH. This MEM formulation contains Earle’s salts for use in a CO2 incubator. This product is made with Earle’s salts.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

This autoclavable form of Gibco™ cell culture medium requires pH adjustment to 4.1–4.2 before autoclaving, following by sodium bicarbonate and L-glutamine supplementation and final pH adjustment to 7.2–7.4 (see protocol for details).

Specifications

Cell Line
HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell Type
Primary Rat Astrocytes
Classification
Animal Origin-free
Form
Powder
Product Type
MEM (Minimum Essential Medium)
Serum Level
Standard Serum Supplementation
Shelf Life
36 Months From Date of Manufacture
Sterility
Sterile-filtered
With Additives
Phenol Red, Earle's Balanced Salt Solution (EBSS)
Without Additives
No HEPES, No Sodium Bicarbonate
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Product Line
Gibco™
Shipping Condition
Room Temperature

Contents & Storage

Storage conditions: 2-8°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture

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