DMEM, high glucose

Citations & References (1)

Gibco™

DMEM, high glucose

Name: DMEM, high glucose
SKU: 11965175

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

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製品番号（カタログ番号）	数量
11965175	10 L
11965092	500 mL
11965118	10 x 500 mL
11965084	1000 mL
11965126	6 x 1000 mL
11965167	5 L

6 オプション

製品番号（カタログ番号） 11965175

価格（JPY）

31,000

キャンペーン価格

Ends: 26-Dec-2025

51,700

割引額 20,700 (40%)

Each

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数量:

10 L

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DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With: High Glucose, L-glutamine, Phenol Red

Without: Sodium Pyruvate, HEPES

The complete formulation is available.

Using DMEM

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

仕様

細胞株HeLa, 293, Cos-7, and PC-12

細胞タイプPrimary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells

濃度1 X

使用対象（アプリケーション）Antibody and cell culture production, Mammalian cell culture

グルコース濃度4500 mg/L

製造品質cGMP-compliant under the ISO 13485 standard

製品ラインGibco, SynerGy

製品タイプDMEM (Dulbecco's Modified Eagle Medium)

数量10 L

品質保持期間12 Months From Date of Manufacture

出荷条件Room Temperature

分類Animal Origin-free

形状Liquid

Serum LevelStandard Serum Supplementation

無菌性Sterile-filtered

Sterilization MethodSterile-filtered

添加剤ありHigh Glucose, Glutamine, Phenol Red

添加剤なしNo HEPES, No Sodium Pyruvate

Unit SizeEach

組成および保存条件

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

よくあるご質問（FAQ）

What is the osmolality of DMEM, high glucose (Cat. No. 11965xxx)?

We do provide osmolality information on the certificate of analysis. All lots of DMEM, high glucose (Cat. No. 11965xxx) will meet the osmolality specification of 320-355 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?

Manganese is not present in the formulation of our catalog DMEM media products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all DMEM, high glucose FAQs

引用および参考文献 (1)

引用および参考文献

Abstract

Plaque production by the polyoma virus.

Authors:DULBECCO R, FREEMAN G,

Journal:Virology

PubMed ID:13669362

Describes the original formulation of DMEM (Dulbecco’s Modified Eagle Medium), used for plaque production by the polyoma virus.