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Gibco™

DMEM, no glucose

Green features
Catalog number: 11966025
Gibco™

DMEM, no glucose

Green features
Catalog number: 11966025
Catalog Number
11966025
also known as 11966-025
Unit Size
500 mL
Price (USD)
Price: 33.89
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
11966025
also known as 11966-025
500 mL
Price: 33.89
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
WithWithout
• L-glutamine• Glucose
• Phenol Red• Sodium Pyruvate
• HEPES

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
For supply chain continuity, we manufacture DMEM at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
1X
Product Type
DMEM (Dulbecco's Modified Eagle Medium)
Sterility
Sterile-filtered
Serum Supplementation
Standard Serum Supplementation
Shipping Condition
Room Temperature
Volume (Metric)
500 ml
Product Line
Gibco™
Form
Liquid
Glutamine
L-Glutamine
Phenol Red Indicator
Phenol Red
Shelf Life
12 Months From Date of Manufacture
Green Features
Sustainable packaging
Cell Type
primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells
With Additives
Phenol Red, L-Glutamine
Without Additives
Glucose, HEPES, Sodium Pyruvate
Glucose
No Glucose
Sodium Pyruvate Additive
No Sodium Pyruvate
Cell Line
HeLa, 293, Cos-7, and PC-12
Classification
Animal Origin-Free
Solution Type
DMEM (Dulbecco's Modified Eagle Medium)
HEPES Buffer
No HEPES

Contents & Storage

Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Media Formulations

Figures