Gibco™

DMEM, high glucose, pyruvate

Green features
Catalog number: 11995123
Gibco™

DMEM, high glucose, pyruvate

Green features
Catalog number: 11995123
Catalog Number
11995123
also known as 11995-123
Unit Size
10 L
Price (USD)
475.00
Your Price:
Availability
***
Quantity
Catalog Number
11995040
also known as 11995-040
Unit Size
1,000 mL
Price (USD)
48.49
Your Price:
Availability
***
Quantity
Catalog Number
11995073
also known as 11995-073
Unit Size
10 x 500 mL
Price (USD)
259.00
Your Price:
Availability
***
Quantity
Catalog Number
11995065
also known as 11995-065
Unit Size
500 mL
Price (USD)
30.11
Your Price:
Availability
***
Quantity
Catalog Number
11995081
also known as 11995-081
Unit Size
6 x 1,000 mL
Price (USD)
249.00
Your Price:
Availability
***
Quantity
Catalog Number
11995115
also known as 11995-115
Unit Size
5 L
Price (USD)
303.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
11995123
also known as 11995-123
10 L
475.00
Your Price:
***
11995040
also known as 11995-040
1,000 mL
48.49
Your Price:
***
11995073
also known as 11995-073
10 x 500 mL
259.00
Your Price:
***
11995065
also known as 11995-065
500 mL
30.11
Your Price:
***
11995081
also known as 11995-081
6 x 1,000 mL
249.00
Your Price:
***
11995115
also known as 11995-115
5 L
303.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
Recommendations
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
WithWithout
• High Glucose• HEPES
• L-glutamine
• Phenol Red
• Sodium Pyruvate

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, Life Technologies offers an identical DMEM product made in our Scotland facility (41966-029). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

Concentration
1X
Product Type
DMEM (Dulbecco's Modified Eagle Medium)
Sterility
Sterile-filtered
Serum Supplementation
Standard Serum Supplementation
Shipping Condition
Ambient
Volume (Metric)
10 L
Product Line
Gibco™, SynerGy™
Form
Liquid
Glutamine
L-Glutamine
Phenol Red Indicator
Phenol Red
Shelf Life
12 Months From Date of Manufacture
Quantity
10L
Cell Type
Primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells
With Additives
High Glucose, L-Glutamine, Phenol Red, Sodium Pyruvate
Without Additives
HEPES
Glucose
High Glucose
Sodium Pyruvate Additive
Sodium Pyruvate
For Use With (Application)
Antibody and cell culture production, Mammalian cell culture
Cell Line
HeLa, 293, Cos-7, and PC-12
Classification
Animal Origin-Free
Solution Type
DMEM (Dulbecco's Modified Eagle Medium)
HEPES Buffer
No HEPES

Contents & Storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Figures