Gateway® pMT-DEST48 Vector

Catalog number: 12282018

Invitrogen™  Related applications: Gateway Cloning | Protein Expression

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Description

The pMT-DEST48 destination vector is designed for rapid cloning with a Gateway® entry clone using lambda phage site-specific recombination and subsequent expression in Drosophila S2 cells. As part of the DES® Expression System, pMT-DEST48 uses the Drosophila metallothionein gene promoter that is induced in S2 cells upon addition of copper sulfate or cadmium chloride to the culture medium. The pMT-DEST48 vector offers the following features:

• C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
• C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
• R sites for efficient recombination with any attL-flanked Gateway® entry vector
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: MT
Vector Type: pMT, pDEST
Product Size: 6 µg
Cloning Method: Gateway®
Protein Tag or Fusion: His Tag (6x), V5 Epitope Tag

Contents & storage

The pMT-DEST48 Gateway® destination vector is supplied supercoiled and lyophilized. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols