Gateway™ pMT-DEST48 Vector

      Catalog number: 12282018

      Invitrogen™  Related applications: Gateway Cloning | Protein Expression

        Contact us for support ›

      Error loading your content!

        Catalog number
      Select a plan
      Unit size
      		 Price ({{currency}}) Availability Qty
      		{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
      		{{product.availability.message}} —
      Pro add-ons
      Customize this product ›
      Check your on-site stock ›

      Your on-site stock

      ›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
      ›› {{supplyCenter.scName}}(Out of stock)
      ›› {{supplyCenter.scName}}
      This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
      Change selected location › Order non-stocked items
      Back to top

      Description

      The pMT-DEST48 destination vector is designed for rapid cloning with a Gateway® entry clone using lambda phage site-specific recombination and subsequent expression in Drosophila S2 cells. As part of the DES® Expression System, pMT-DEST48 uses the Drosophila metallothionein gene promoter that is induced in S2 cells upon addition of copper sulfate or cadmium chloride to the culture medium. The pMT-DEST48 vector offers the following features:

      • C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
      • C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
      • R sites for efficient recombination with any attL-flanked Gateway® entry vector
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Cloning Method: Gateway®
      Product Line: DES®, Gateway®
      Product Size: 6 µg
      Promoter: MT
      Protein Tag or Fusion: His Tag (6x), V5 Epitope Tag
      Vector Type: pDEST, pMT

      Contents & storage

      The pMT-DEST48 Gateway® destination vector is supplied supercoiled and lyophilized. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.

      Documents

      Manuals & protocols