Invitrogen™

Gateway™ pMT-DEST48 Vector

Catalog number:  12282018

Related applications:

Protein Expression

| Gateway Cloning

Back to top

Description

The pMT-DEST48 destination vector is designed for rapid cloning with a Gateway® entry clone using lambda phage site-specific recombination and subsequent expression in Drosophila S2 cells. As part of the DES® Expression System, pMT-DEST48 uses the Drosophila metallothionein gene promoter that is induced in S2 cells upon addition of copper sulfate or cadmium chloride to the culture medium. The pMT-DEST48 vector offers the following features:

• C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
• C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
• R sites for efficient recombination with any attL-flanked Gateway® entry vector
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Vector: pMT, pDEST
Promoter: MT
Product Line: DES™, Gateway™
Protein Tag: His Tag (6x), V5 Epitope Tag
Cloning Method: Gateway™

Contents & storage

The pMT-DEST48 Gateway™ destination vector is supplied supercoiled and lyophilized. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.