AMV Reverse Transcriptase, cloned

Catalog number: 12328019

Invitrogen™  Related applications: Reverse Transcription

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AMV Reverse Transcriptase, Cloned, is used to synthesize first-strand cDNA for amplification by PCR or for cloning subsequent to conversion to double-stranded cDNA. Cloned Avian Myeloblastosis Virus (AMV) Reverse Transcriptase (RT) is highly purified from insect cells infected with baculovirus containing the pol gene of AMV. The resulting enzyme has a higher specific activity than does native AMV RT, providing enhanced cDNA yields and improved sensitivity. The enzyme, accompanying buffers, and protocols are optimized to synthesize first-strand cDNA for amplification by PCR, and for cloning double-stranded cDNA of up to 12 kb. AMV RT has endoribonuclease H activity.

Features of this enzyme:

Flexibility—works with oligo(dT), random primers, or gene-specific primers
Thermostability—AMV is active at temperatures up to 55°C
Multiple applications—first-strand cDNA synthesis for RT-PCR, and sequencing single- and double-stranded DNA and RNA

Purified from insect cells infected with recombinant baculovirus expressing AMV pol.

Unit definition
One unit of cloned AMV RT is the amount of enzyme required to incorporate 1 nmole of dexoyribonucleotide into acid-precipitable material in 10 minutes at 37°C using poly(A)oligo(dT)25 as template-primer.
For Research Use Only. Not for use in diagnostic procedures.


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Optimal Reaction Temperature: Up to 55° C
Final Product Size(s): 12.3 kb or less
Reverse Transcriptase: AMV (Recombinant)
Ribonuclease H Activity: Yes
Format: Tube(s)
Quantity: 750 units
Template: ssRNA
Sensitivity: Low
Product Size: 750 units
Concentration: 15 U&⁄µl
Enzyme Function: RNA dependent DNA Polymerase
Shipping Condition: Dry Ice
Number of Reactions: 50 Reactions

Contents & storage

• Cloned AMV RT (50 µL at 15U/µL)
• 1 vial of 5X cDNA synthesis buffer [250 mM Tris-acetate pH 8.4, 375 mM potassium acetate, 40 mM magnesium acetate, and stabilizers]
• 1 vial of 100 mM DTT
• 1 vial of 40 mM magnesium acetate
• 1 vial of 40 mM sodium pyrophosphate

Store at -20°C. Stable for 12 months when stored at -20°C. Stability extended by storing at -70°C.


Manuals & protocols

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