Search

  • Auftragsstatus
  • Eilbestellung
  • Aktionen

Cloned AMV First-Strand cDNA Synthesis Kit - FAQs

View additional product information for Cloned AMV First-Strand cDNA Synthesis Kit - FAQs (12328040)

2 product FAQs found

How long can I store the cDNA from my reverse transcription step?

You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.

Why do you add sodium pyrophosphate and/or spermidine into the reaction mixture with the AMV reverse transcriptase in the Cloned AMV First-Strand cDNA Synthesis Kit?

Both sodium pyrophosphate and spermidine help reduce the secondary structure of the RNA. Although one or the other will take care of the majority of the secondary structure of most RNAs, adding both ensures the removal of the most resilient RNA secondary structure.