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Invitrogen™

AccuPrime™ GC-Rich DNA Polymerase

Catalog number: 12337016
Invitrogen™

AccuPrime™ GC-Rich DNA Polymerase

Catalog number: 12337016
Catalog Number
12337016
also known as 12337-016
Unit Size
200 rxns
Price (USD)
Price: 590.00
Online price: 567.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
1,000 reactions
Price (USD)
Price: 2,585.00
Online price: 2,337.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12337016
also known as 12337-016
200 rxns
Price: 590.00
Online price: 567.65
Your price:
-
123370241,000 reactions
Price: 2,585.00
Online price: 2,337.65
Your price:
-
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AccuPrime™ GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B).

Benefits of using AccuPrime™ GC-Rich DNA Polymerase:
Yield—high yields for targets up to 5 kb in length
Specificity—AccuPrime™ accessory proteins for improved PCR specificity
Sensitivity—sensitivity down to 5 ng of template DNA

Robust and specific amplifications
The AccuPrime™ buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.

Unit definition
One unit of AccuPrime™ GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble material in 30 min at 74°C.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

GC-Rich PCR Performance
High
Polymerase
AccuPrime GC-Rich DNA Polymerase
Reaction Speed
Standard
Shipping Condition
Wet or Dry Ice
For Use With (Application)
Hot-start PCR
Fidelity (vs. Taq)
2X
Hot Start
Built-In Hot Start
No. of Reactions
200 Reactions
Overhang
Blunt
Reaction Format
Separate Components
Size (Final Product)
5 kb or less
Volume
100 μL

Contents & Storage

• AccuPrime GC-Rich DNA Polymerase (1 x 100 μL at 2 U/μL)
• 5X AccuPrime GC-Rich Buffer A (1 x 1 mL)
• 5X AccuPrime GC-Rich Buffer B (1 x 1 mL)
• 50 mM MgSO4 (1 x 1 mL)

Store at –20°C.

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