AccuPrime™ SuperMix I

      AccuPrime™ SuperMix I

      Numéro de catalogue:  12342010

      Invitrogen™  Applications liées: Methylation Analysis | PCR

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      Description

      AccuPrime™ SuperMix I is a ready-to-use mixture of DNA polymerase, accesory proteins, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half (Figure 1).

      For accurate PCR performance, AccuPrime™ SuperMix I sets the standard. You'll amplify your DNA targets cycle after cycle, eliminating troubleshooting and rework. In addition to a powerful DNA polymerase, AccuPrime™ SuperMix I includes Platinum® anti-DNA polymerase antibodies that inhibit polymerase activity, providing an automatic hot-start, and a thermal stable accessory protein that enhances specific primer-template hybridization during every cycle of PCR (Figure 1). This unique combination allows for success, even under previously suboptimal conditions.

      AccuPrime™ SuperMix I is designed for amplification of genomic DNA amplicons (?200 bp), plasmid DNA, or cDNA templates. Alternatively, AccuPrime™ SuperMix II is designed to amplify a broader range of amplicon sizes up to 4kb.
      For Research Use Only. Not for use in diagnostic procedures.

      Spécifications

      Polymerase: AccuPrime™ Taq DNA Polymerase
      Hot Start: Built-In Hot Start
      Fidelity (vs. Taq): 2 X
      GC-Rich PCR Performance: Low
      Reaction Format: SuperMix or Master Mix
      Product Overhang: 3'-A
      Reaction Speed: Standard
      Final Product Size(s): 200 bp or less
      Exonuclease Activity: 5' - 3'
      Product Line: AccuPrime™
      Product Size: 200 reactions
      Shipping Condition: Dry Ice

      Contenu et stockage

      AccuPrime™ SuperMix I is supplied at 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 200 amplification reactions of 25 µl each are provided.

      AccuPrime™ SuperMix I Components:
      40 mM Tris-HCl (pH 8.4), 100 mM KCl, 3 mM MgCl2, 400 µM dGTP, 400 µM dATP, 400 µM dTTP, 400 µM dCTP, AccuPrime™ Taq DNA Polymerase, thermostable AccuPrime™ protein, stabilizers.

      200 rxns: 2 × 1.25 ml

      Store at -20°ree;C in a non-frost-free freezer.

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