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AccuPrime™ Pfx DNA Polymerase
AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis.
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| Catalog Number | No. of Reactions |
|---|---|
| 12344024 | 200 Reactions |
| 12344032 | 1000 Reactions |
Catalog number 12344024
Price (USD)/ Each
568.65
Online exclusive
598.00 Save 29.35 (5%)
-
No. of Reactions:
200 Reactions
Price (USD)/ Each
568.65
Online exclusive
598.00 Save 29.35 (5%)
AccuPrime™ Pfx DNA Polymerase
Catalog number12344024
Price (USD)/ Each
568.65
Online exclusive
598.00 Save 29.35 (5%)
-
AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3'→5' exonuclease) activity. Platinum anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum Pfx alone.
Benefits of AccuPrime Pfx DNA Polymerase
- Greater than 26 times higher fidelity than Taq DNA polymerase
- Amplification of fragments up to 12 kb
- Minimized PCR optimization
Applications
- Amplification of DNA from complex genomic, viral, and plasmid templates
- RT-PCR
- High-fidelity PCR
- Hot-start PCR
Notes
- AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.
- The 10X AccuPrime Pfx reaction mix contains MgSO4, dNTPs, and thermostable proteins that enhance specific primer-template hybridization during each PCR cycle.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodPrimer-probe
GC-Rich PCR PerformanceLow
PolymeraseAccuPrime Pfx DNA Polymerase
Reaction SpeedStandard
Quantity200 Reactions
Shipping ConditionWet or Dry Ice
For Use With (Application)Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq)26X
Hot StartBuilt-In Hot Start
No. of Reactions200 Reactions
OverhangBlunt
Reaction FormatSeparate Components
Size (Final Product)12 kb or less
Volume100 μL
Unit SizeEach
Contents & Storage
• AccuPrime Pfx DNA Polymerase (1 x 100 μL at 2.5 U/μL)
• 10X AccuPrime Pfx Reaction Mix (1 x 1 mL)
• 50 mM MgSO4 (1 x 1 mL)
Store at -20°C.
• 10X AccuPrime Pfx Reaction Mix (1 x 1 mL)
• 50 mM MgSO4 (1 x 1 mL)
Store at -20°C.