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AccuPrime™ Pfx DNA Polymerase

Catalog number:  12344024

 Related applications:

Methylation Analysis


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AccuPrime™ Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3´→5´ exonuclease) activity. Platinum® anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). Thermostable AccuPrime™ accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum® Pfx alone. With AccuPrime™ Pfx DNA Polymerase you will:

• Achieve greater than 26 times higher fidelity than Taq DNA polymerase
• Amplify fragments up to 12 kb
• Minimize PCR optimizationApplications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. AccuPrime™ Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.
For Research Use Only. Not for use in diagnostic procedures.


Polymerase: AccuPrime™ Pfx DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 26 X
GC-Rich PCR Performance: Low
Reaction Format: Separate Components
Product Overhang: Blunt
Reaction Speed: Standard
Final Product Size(s): 12 kb or less
Exonuclease Activity: 3' - 5'
Product Line: AccuPrime™
Product Size: 200 reactions
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

• 1 x 100 μL AccuPrime Pfx DNA Polymerase (2.5 U/µl)
• 1 x 1 mL 10X AccuPrime Pfx Reaction Mix
• 1 x 1 mL 50 mM MgSO4

Store at -20°C.


Manuals & protocols