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      AccuPrime™ Taq DNA Polymerase, high fidelity

      AccuPrime™ <i>Taq</i> DNA Polymerase, high fidelity

      Numéro de catalogue:  12346086

      Invitrogen™  Applications liées: Methylation Analysis | PCR

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      Description

      Invitrogen AccuPrime Taq DNA Polymerase, High Fidelity, provides qualified reagents for the high-fidelity PCR amplification of nucleic acid templates. It includes an enzyme blend of Platinum Taq DNA Polymerase and a proofreading enzyme, plus AccuPrime accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases.

      With AccuPrime Taq DNA Polymerase, High Fidelity, you'll get:
      • The highest specificity and yield for the most robust PCR amplification
      • 9-fold higher fidelity than Taq DNA polymerase alone
      • Mimimal optimization steps, even with non-optimized primer sets
      • Efficient amplification of targets over a broad size range up to 20 kb

      How it works
      Pyrococcus species GB-D polymerase is a proofreading enzyme that possesses a 3´→5´ exonuclease activity. Mixing this enzyme with Taq DNA Polymerase increases fidelity and allows amplification of simple and complex DNA templates over a large range of target sizes.

      The Platinum antibody complexes with Taq DNA polymerase and inhibits activity at room temperature. Activity is restored after the initial denaturation step, providing an automatic hot start PCR.

      The thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, preventing mispriming and enhancing PCR specificity and yield.

      For Research Use Only. Not for use in diagnostic procedures.

      Spécifications

      Polymerase: AccuPrime™ Taq DNA Polymerase High Fidelity
      Hot Start: Built-In Hot Start
      Fidelity (vs. Taq): 9 X
      GC-Rich PCR Performance: Low
      Reaction Format: Separate Components
      Product Overhang: Mixed
      Reaction Speed: Standard
      Final Product Size(s): 20 kb or less
      Exonuclease Activity: 3' - 5', 5' - 3'
      Product Line: AccuPrime™
      Product Size: 200 reactions
      Shipping Condition: Dry Ice

      Contenu et stockage

      AccuPrime™ Taq DNA Polymerase High Fidelity (40 µL, at 5U/µL) is supplied with:
      • 50 mM MgSO4 (1 mL)
      • 10X AccuPrime™ PCR Buffer I (1 mL)
      • 10X AccuPrime™ PCR Buffer II (1 mL)

      Buffer I and II differ in their concentration of thermostable AccuPrime™ protein. Their components are: 600 mM Tris-SO4 (pH 8.9), 180 mM (NH4)2SO4, 20 mM MgSO4, 2 mM dGTP, 2 mM dATP, 2 mM dTTP, 2 mM dCTP, thermostable AccuPrime™ protein, 10% glycerol. The supplied PCR Buffer is a 10X concentrate and should be diluted for use. Buffer I is optimized for plasmids, cDNA, and λ DNA. Buffer II is optimized for genomic DNA.

      Store at -30°C to -10°C.

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