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Invitrogen™

AccuPrime™ Taq DNA Polymerase, high fidelity

Catalog number:  12346086

 Related applications: Methylation Analysis | PCR

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Description

Invitrogen AccuPrime Taq DNA Polymerase, High Fidelity, provides qualified reagents for the high-fidelity PCR amplification of nucleic acid templates. It includes an enzyme blend of Platinum Taq DNA Polymerase and a proofreading enzyme, plus AccuPrime accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases.

With AccuPrime Taq DNA Polymerase, High Fidelity, you'll get:
• The highest specificity and yield for the most robust PCR amplification
• 9-fold higher fidelity than Taq DNA polymerase alone
• Mimimal optimization steps, even with non-optimized primer sets
• Efficient amplification of targets over a broad size range up to 20 kb

How it works
Pyrococcus species GB-D polymerase is a proofreading enzyme that possesses a 3´→5´ exonuclease activity. Mixing this enzyme with Taq DNA Polymerase increases fidelity and allows amplification of simple and complex DNA templates over a large range of target sizes.

The Platinum antibody complexes with Taq DNA polymerase and inhibits activity at room temperature. Activity is restored after the initial denaturation step, providing an automatic hot start PCR.

The thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, preventing mispriming and enhancing PCR specificity and yield.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: AccuPrime™ Taq DNA Polymerase High Fidelity
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 9 X
GC-Rich PCR Performance: Low
Reaction Format: Separate Components
Product Overhang: Mixed
Reaction Speed: Standard
Final Product Size(s): 20 kb or less
Exonuclease Activity: 3' - 5', 5' - 3'
Product Line: AccuPrime™
Product Size: 200 reactions
Shipping Condition: Dry Ice

Contents & storage

AccuPrime™ Taq DNA Polymerase High Fidelity (40 µL, at 5U/µL) is supplied with:
• 50 mM MgSO4 (1 mL)
• 10X AccuPrime™ PCR Buffer I (1 mL)
• 10X AccuPrime™ PCR Buffer II (1 mL)

Buffer I and II differ in their concentration of thermostable AccuPrime™ protein. Their components are: 600 mM Tris-SO4 (pH 8.9), 180 mM (NH4)2SO4, 20 mM MgSO4, 2 mM dGTP, 2 mM dATP, 2 mM dTTP, 2 mM dCTP, thermostable AccuPrime™ protein, 10% glycerol. The supplied PCR Buffer is a 10X concentrate and should be diluted for use. Buffer I is optimized for plasmids, cDNA, and λ DNA. Buffer II is optimized for genomic DNA.

Store at -30°C to -10°C.

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