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AccuPrime™ Taq DNA Polymerase, high fidelity

Catalog number:  12346086

Invitrogen™  Related applications: Methylation Analysis | PCR

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AccuPrime™ Taq DNA Polymerase, High Fidelity, amplifies nucleic acid templates using antibody-mediated hot-start, a blend of Taq DNA Polymerase and a proofreading enzyme, and AccuPrime™ accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases. With AccuPrime™ Taq DNA Polymerase, High Fidelity, you'll get:

• The highest specificity and yield for the most robust PCR amplification
• 9-fold higher fidelity than Taq DNA polymerase alone
• Mimimal optimization steps, even with non-optimized primer sets
• Efficient amplification of targets over a broad size range up to 20 kbHow it works
High fidelity is achieved by a combination of Platinum® anti-Taq DNA polymerase antibodies that inhibit polymerase activity, providing an automatic "hot-start", and the proofreading (3´→5´ exonuclease activity) enzyme Pyrococcus species GB-D. The thermostable AccuPrime™ accessory proteins enhance specific primer-template hybridization during every cycle of PCR, preventing mispriming and enhancing PCR specificity and yield.
For Research Use Only. Not for use in diagnostic procedures.


Polymerase: AccuPrime™ Taq DNA Polymerase High Fidelity
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 9 X
GC-Rich PCR Performance: Low
Reaction Format: Separate Components
Product Overhang: Mixed
Reaction Speed: Standard
Final Product Size(s): 20 kb or less
Exonuclease Activity: 3' - 5', 5' - 3'
Product Line: AccuPrime™
Product Size: 200 reactions
Shipping Condition: Dry Ice

Contents & storage

AccuPrime™ Taq DNA Polymerase High Fidelity (40 µL, at 5U/µL)) is supplied with:
• 50 mM MgSO4 (1 mL)
• 10X AccuPrime™ PCR Buffer I (1 mL)
• 10X AccuPrime™ PCR Buffer II (1 mL)

Buffer I and II differ in their concentration of thermostable AccuPrime™ protein. Their components are: 600 mM Tris-SO4 (pH 8.9), 180 mM (NH4)2SO4, 20 mM MgSO4, 2 mM dGTP, 2 mM dATP, 2 mM dTTP, 2 mM dCTP, thermostable AccuPrime™ protein, 10% glycerol. The supplied PCR Buffer is a 10X concentrate and should be diluted for use. Buffer I is optimized for plasmids, cDNA, and λ DNA. Buffer II is optimized for genomic DNA.

Store at -30°C to -10°C.


Manuals & protocols

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