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Platinum® PCR SuperMix High Fidelity

Catalog number: 12532016

Invitrogen™  Related applications: PCR

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Platinum® PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. You need only add template and primers, reducing set-up time by half (see figure). With Platinum® PCR SuperMix, High Fidelity, you'll:

• Achieve greater than six-times higher fidelity than Taq DNA polymerase
• Amplify fragments up to 15 kb
• Produce products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs
• Minimize PCR optimization

Using Platinum® PCR SuperMix, High Fidelity
Platinum® PCR SuperMix, High Fidelity, is for high-specificity, high-fidelity DNA amplification applications, such as cloning or mutagenesis. High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic "hot-start" PCR. This hot-start capability increases specificity and yield and allows for room-temperature reaction assembly.
For Research Use Only. Not for use in diagnostic procedures.


Polymerase: Platinum® Taq DNA Polymerase High Fidelity
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 6 X
GC-Rich PCR Performance: Low
Reaction Format: SuperMix or Master Mix
Product Overhang: Mixed
Reaction Speed: Standard
Final Product Size(s): 15 kb or less
Product Size: 100 reactions
Exonuclease Activity: 5' - 3', 3' - 5'
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

Platinum® PCR SuperMix High Fidelity is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 100 amplification reactions of 50 µl each are provided (4 × 1.125 mL).

Platinum® PCR SuperMix High Fidelity Components: 22 U/ml complexed recombinant Taq DNA polymerase, Pyrococcus species GB-D thermostable polymerase, and Platinum® Taq Antibody; 66 mM Tris-SO4 (pH 8.9); 19.8 mM (NH4)2SO4; 2.4 mM MgSO4; 220 µM dNTPs; and stabilizers.

Store at -20°C in a non-frost-free freezer.


Manuals & protocols

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