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Invitrogen™

Platinum™ PCR SuperMix High Fidelity

Catalog number: 12532016
Invitrogen™

Platinum™ PCR SuperMix High Fidelity

Catalog number: 12532016
Catalog Number
12532016
also known as 12532-016
Unit Size
100 reactions
Price (USD)
322.00
Your Price:
Availability
***
Quantity
Catalog Number
12532024
also known as 12532-024
Unit Size
5,000 reactions
Price (USD)
11,640.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12532016
also known as 12532-016
100 reactions
322.00
Your Price:
***
12532024
also known as 12532-024
5,000 reactions
11,640.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Platinum® PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. You need only add template and primers, reducing set-up time by half (see figure). With Platinum® PCR SuperMix, High Fidelity, you'll:

• Achieve greater than six-times higher fidelity than Taq DNA polymerase
• Amplify fragments up to 15 kb
• Produce products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs
• Minimize PCR optimization

Using Platinum® PCR SuperMix, High Fidelity
Platinum® PCR SuperMix, High Fidelity, is for high-specificity, high-fidelity DNA amplification applications, such as cloning or mutagenesis. High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic 'hot-start' PCR. This hot-start capability increases specificity and yield and allows for room-temperature reaction assembly.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Exonuclease Activity
3' - 5', 5' - 3'
Product Line
Platinum™
Quantity
100 reactions
Shipping Condition
Approved for shipment on Wet or Dry Ice
Fidelity (vs. Taq)
6 X
Form
Liquid
GC-Rich PCR Performance
Low
Hot Start
Built-In Hot Start
No. of Reactions
100 Reactions
Overhang
Mixed
Polymerase
Platinum™ Taq DNA Polymerase High Fidelity
Reaction Format
SuperMix or Master Mix
Reaction Speed
Standard
Size (Final Product)
15 kb or less
Volume
1.125 mL

Contents & Storage

• 4 × 1.125 mL Platinum PCR SuperMix High Fidelity

Store at -20°C in a non-frost-free freezer.

Figures

Frequently asked questions (FAQs)

Citations & References