Platinum® PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. You need only add template and primers, reducing set-up time by half (see figure). With Platinum® PCR SuperMix, High Fidelity, you'll:
• Achieve greater than six-times higher fidelity than Taq DNA polymerase • Amplify fragments up to 15 kb • Produce products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs • Minimize PCR optimization
Using Platinum® PCR SuperMix, High Fidelity Platinum® PCR SuperMix, High Fidelity, is for high-specificity, high-fidelity DNA amplification applications, such as cloning or mutagenesis. High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic 'hot-start' PCR. This hot-start capability increases specificity and yield and allows for room-temperature reaction assembly.
For Research Use Only. Not for use in diagnostic procedures.
3' - 5', 5' - 3'
Approved for shipment on Wet or Dry Ice
Fidelity (vs. Taq)
GC-Rich PCR Performance
Built-In Hot Start
No. of Reactions
Platinum™ Taq DNA Polymerase High Fidelity
SuperMix or Master Mix
Size (Final Product)
15 kb or less
Contents & Storage
• 4 × 1.125 mL Platinum PCR SuperMix High Fidelity