pIB/V5-His-DEST Vector

Catalog number: 12550018

Invitrogen™  Related applications: Gateway Cloning | Protein Expression

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Description

The pIB/V5-His-DEST vector is designed for rapid lambda phage site-specific recombination with a Gateway® entry clone and subsequent expression in Sf9, Sf21, or High Five™ insect cells. As part of the InsectSelect™ System, pIB/V5-His-DEST features:

• The OpIE2 promoter for constitutive expression
• Blasticidin resistance gene driven by the weak GP64 promoter for selection of stable cell lines that have integrated the plasmid into a transcriptionally active site
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and easy purification with a nickel-chelating resin
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Promoter: OplE2
Vector Type: pIB, pDEST
Product Size: 6 µg
Cloning Method: Gateway®
Protein Tag or Fusion: His Tag (6x), V5 Epitope Tag
Selection Agent (Eukaryotic): Blasticidin

Contents & storage

The vectors are provided supercoiled and lyophilized and include 6 µg of expression vector and 10 µg of an expression control. The vectors are guaranteed stable for 6 months when stored properly.

Documents

Manuals & protocols