pIB/V5-His-DEST Vector

Catalog number:  12550018

 Related applications:

Gateway Cloning

| Protein Expression

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


The pIB/V5-His-DEST vector is designed for rapid lambda phage site-specific recombination with a Gateway® entry clone and subsequent expression in Sf9, Sf21, or High Five™ insect cells. As part of the InsectSelect™ System, pIB/V5-His-DEST features:

• The OpIE2 promoter for constitutive expression
• Blasticidin resistance gene driven by the weak GP64 promoter for selection of stable cell lines that have integrated the plasmid into a transcriptionally active site
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and easy purification with a nickel-chelating resin
For Research Use Only. Not for use in diagnostic procedures.



Cloning Method: Gateway®
Product Line: Gateway®, InsectSelect™
Product Size: 6 µg
Promoter: OplE2
Protein Tag or Fusion: His Tag (6x), V5 Epitope Tag
Selection Agent (Eukaryotic): Blasticidin
Vector Type: pDEST, pIB

Contents & storage

The vectors are provided supercoiled and lyophilized and include 6 µg of expression vector and 10 µg of an expression control. The vectors are guaranteed stable for 6 months when stored properly.


Manuals & protocols