SuperScript® III One-Step RT-PCR System with Platinum® Taq High Fidelity DNA Polymerase

Catalog number: 12574030

Invitrogen™  Related applications: Reverse Transcription

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Description

The SuperScript® III One-Step RT-PCR System with Platinum® Taq DNA Polymerase offers our highest sensitivity available for end point detection. This system combines the high thermostability of SuperScript® III RT with the specificity of Platinum® Taq DNA Polymerase High Fidelity to increase priming specificity, generate higher product yields, and identify a larger range of targets. The one-step format enables easy analysis of gene expression or detection of rare or viral RNA.
Sensitivity - Routine detection down to 1 pg total RNA
Convenience - Single-tube format for speed, convenience, and less reaction-to-reaction variability. Compatible with multiplexing
Specificity - Uses SuperScript® III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers
Amplicon Size - Detection of targets from 300 bp to 10 kb for greater flexibility (Figure 1)

High-Fidelity PCR Amplification
Platinum® Taq DNA Polymerase High Fidelity is ideal for the amplification of DNA fragments, when high yields and robust amplification are required. Fidelity is maximized through the use of a mixture of Platinum® Taq DNA Polymerase and the proofreading (3’-5’ exonuclease activity) enzyme Pyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum® automatic "hot-start" technology.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

PCR Method: 1-Step RT-PCR
Reverse Transcriptase: SuperScript® III
Polymerase: Platinum® Taq DNA Polymerase High Fidelity
Hot Start: Built-In Hot Start
Sample Type (General): RNA
Product Size: 25 reactions
Detection Method: Gel Electrophoresis
Shipping Condition: Dry Ice
Final Product Size(s): 10 kb or less
Primer-Probe Compatibility: Unlabeled Oligos

Contents & storage

Components:
• 25 µL SuperScript® III RT/Platinum® Taq High Fidelity Enzyme Mix
• 1 mL 2X Reaction Mix (containing 0.4 mM of each dNTP, 2.4 mM MgSO4)
• 500 µL 5 mM Magnesium Sulfate

Store all components at -30°C to -10°C.

Documents

Manuals & protocols

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