To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available.
Additional materials required, available separately: Gateway™ entry clone, Gateway™ LR Clonase™ enzyme mix, and reaction buffer.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible System
Inducible
Inducing Agent
IPTG
Promoter
T7
Product Type
Expression Kit
Selection Agent (Eukaryotic)
None
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Bacterial or Yeast Strain
BL21 Star™(DE3), TOP10
Detection Location
In-Gel Detection
Expression System
E. coli
Expression Mechanism
Cell-Based Expression
Protein Tag
His Tag (6x), Lumio
Cloning Method
Gateway
Vector
pET
Product Line
Champion™, Gateway™, Lumio™
Contents & Storage
All destination vectors are provided lyophilized and supercoiled.