SuperScript™ IV One-Step RT-PCR System

Description

The Invitrogen SuperScript IV One-Step RT-PCR System combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance. With this convenient one-step formulation plus gene-specific primers, you can perform both cDNA synthesis from total RNA or mRNA and PCR amplification in a single tube.

Features of the SuperScript IV One-Step RT-PCR System include:
• Superior performance with sensitivity down to 0.01 pg RNA, target length up to 13.8 kb, and the fastest one-step RT-PCR protocol
• Reliable target detection even with suboptimally pure RNA samples
• Two-phase hot-start activation mechanism for high sensitivity, specificity, and room temperature setup

SuperScript IV RT is a proprietary MMLV RT mutant with increased processivity, thermostability, and inhibitor resistance, as well as reduced RNase H activity, for sensitive and efficient full-length cDNA synthesis. Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. The combination of these two engineered enzymes produces enhanced RT-PCR yields and fidelity, as well as amplification of longer templates compared to other one-step RT-PCR kits.

For even better performance, we offer SuperScript IV One-Step RT-PCR System with ezDNase. The ezDNase further enhances the one-step RT-PCR workflow through an extremely simplified genomic DNA removal step that ensures the highest accuracy and confidence in one-step RT-PCR results.

Specifications

Hot Start:	Built-In Hot Start
Product Line:	SuperScript™
Reverse Transcriptase:	SuperScript™ IV
Sample Type:	RNA
Shipping Condition:	Dry Ice
Detection Method:	Gel Electrophoresis
Format:	Tube
GC-Rich PCR Performance:	High
No. of Reactions:	25
No. of Tests:	25 Reactions
PCR Method:	1-Step RT-PCR
Polymerase:	Platinum™ SuperFi™ DNA Polymerase
Product Type Specs:	One-Step RT-PCR System
Reaction Speed:	Fast
Size (Final Product):	14 kb or less
Sufficient For:	25 Reactions
Volume:	12.2 μL

Contents & storage