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Invitrogen™

SuperScript™ IV One-Step RT-PCR System

Catalog number: 12594025
Invitrogen™

SuperScript™ IV One-Step RT-PCR System

Catalog number: 12594025
The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance. With this convenient one-step formulation plus gene-specific primers, you can perform both cDNA synthesis from total RNA or mRNA and PCR amplification in a single tube.
 
Catalog Number
12594025
Unit Size
Each
Includes
Kit only
No. of Reactions
25 Reactions
Price (USD)
Price: 370.00
Online price: 345.65
Your price:
Availability
-
Full specifications
Quantity
Detection MethodGel Electrophoresis
FormatKit
GC-Rich PCR PerformanceHigh
PCR Method1-step RT-PCR
PolymerasePlatinum SuperFi
Reaction Speed10 min.
Technique1-step RT-PCR
Optimal Reaction Temperature45°C to 60°C
Reverse TranscriptaseSuperScript IV
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
Final Product TypePCR Amplified cDNA
Hot StartBuilt-In Hot Start
No. of Reactions25 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 14 kb
Starting MaterialRNA
IncludesKit only
Unit SizeEach
Catalog Number
Unit Size
Each
Includes
Kit only
No. of Reactions
100 Reactions
Price (USD)
Price: 1,044.00
Online price: 970.65
Your price:
Availability
-
Full specifications
Quantity
Detection MethodGel Electrophoresis
FormatKit
GC-Rich PCR PerformanceHigh
PCR Method1-step RT-PCR
PolymerasePlatinum SuperFi
Reaction Speed10 min.
Technique1-step RT-PCR
Optimal Reaction Temperature45°C to 60°C
Reverse TranscriptaseSuperScript IV
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
Final Product TypePCR Amplified cDNA
Hot StartBuilt-In Hot Start
No. of Reactions100 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 14 kb
Starting MaterialRNA
IncludesKit only
Unit SizeEach
Catalog Number
Unit Size
Each
Includes
Kit with ezDNase
No. of Reactions
25 Reactions
Price (USD)
Price: 405.00
Online price: 399.65
Your price:
Availability
-
Full specifications
Quantity
Detection MethodGel Electrophoresis
FormatKit
GC-Rich PCR PerformanceHigh
PCR Method1-step RT-PCR
PolymerasePlatinum SuperFi
Reaction Speed10 min.
Technique1-step RT-PCR
Optimal Reaction Temperature45°C to 60°C
Reverse TranscriptaseSuperScript IV
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
Final Product TypePCR Amplified cDNA
Hot StartBuilt-In Hot Start
No. of Reactions25 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 14 kb
Starting MaterialRNA
IncludesKit with ezDNase
Unit SizeEach
Catalog Number
Unit Size
Each
Includes
Kit with ezDNase
No. of Reactions
100 Reactions
Price (USD)
Price: 1,150.00
Online price: 1,135.65
Your price:
Availability
-
Full specifications
Quantity
Detection MethodGel Electrophoresis
FormatKit
GC-Rich PCR PerformanceHigh
PCR Method1-step RT-PCR
PolymerasePlatinum SuperFi
Reaction Speed10 min.
Technique1-step RT-PCR
Optimal Reaction Temperature45°C to 60°C
Reverse TranscriptaseSuperScript IV
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
Final Product TypePCR Amplified cDNA
Hot StartBuilt-In Hot Start
No. of Reactions100 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 14 kb
Starting MaterialRNA
IncludesKit with ezDNase
Unit SizeEach
Showing 4 of 4
Catalog NumberSpecificationsUnit SizeIncludesNo. of ReactionsPrice (USD)AvailabilityQuantity
12594025Full specifications
Each Kit only25 Reactions
Price: 370.00
Online price: 345.65
Your price:
-
Detection MethodGel Electrophoresis
FormatKit
GC-Rich PCR PerformanceHigh
PCR Method1-step RT-PCR
PolymerasePlatinum SuperFi
Reaction Speed10 min.
Technique1-step RT-PCR
Optimal Reaction Temperature45°C to 60°C
Reverse TranscriptaseSuperScript IV
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
Final Product TypePCR Amplified cDNA
Hot StartBuilt-In Hot Start
No. of Reactions25 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 14 kb
Starting MaterialRNA
IncludesKit only
Unit SizeEach
12594100Full specifications
Each Kit only100 Reactions
Price: 1,044.00
Online price: 970.65
Your price:
-
Detection MethodGel Electrophoresis
FormatKit
GC-Rich PCR PerformanceHigh
PCR Method1-step RT-PCR
PolymerasePlatinum SuperFi
Reaction Speed10 min.
Technique1-step RT-PCR
Optimal Reaction Temperature45°C to 60°C
Reverse TranscriptaseSuperScript IV
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
Final Product TypePCR Amplified cDNA
Hot StartBuilt-In Hot Start
No. of Reactions100 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 14 kb
Starting MaterialRNA
IncludesKit only
Unit SizeEach
12595025Full specifications
Each Kit with ezDNase25 Reactions
Price: 405.00
Online price: 399.65
Your price:
-
Detection MethodGel Electrophoresis
FormatKit
GC-Rich PCR PerformanceHigh
PCR Method1-step RT-PCR
PolymerasePlatinum SuperFi
Reaction Speed10 min.
Technique1-step RT-PCR
Optimal Reaction Temperature45°C to 60°C
Reverse TranscriptaseSuperScript IV
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
Final Product TypePCR Amplified cDNA
Hot StartBuilt-In Hot Start
No. of Reactions25 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 14 kb
Starting MaterialRNA
IncludesKit with ezDNase
Unit SizeEach
12595100Full specifications
Each Kit with ezDNase100 Reactions
Price: 1,150.00
Online price: 1,135.65
Your price:
-
Detection MethodGel Electrophoresis
FormatKit
GC-Rich PCR PerformanceHigh
PCR Method1-step RT-PCR
PolymerasePlatinum SuperFi
Reaction Speed10 min.
Technique1-step RT-PCR
Optimal Reaction Temperature45°C to 60°C
Reverse TranscriptaseSuperScript IV
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
Final Product TypePCR Amplified cDNA
Hot StartBuilt-In Hot Start
No. of Reactions100 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 14 kb
Starting MaterialRNA
IncludesKit with ezDNase
Unit SizeEach
Showing 4 of 4

Features of the SuperScript IV One-Step RT-PCR System include:
• Superior performance with sensitivity down to 0.01 pg RNA, target length up to 13.8 kb, and the fastest one-step RT-PCR protocol
• Reliable target detection even with suboptimally pure RNA samples
• Two-phase hot-start activation mechanism for high sensitivity, specificity, and room temperature setup
• Fast and easy gDNA removal for superior accuracy and confidence in results (SuperScript IV One-Step RT-PCR System with ezDNase only)

SuperScript IV RT is a proprietary MMLV RT mutant with increased processivity, thermostability, and inhibitor resistance, as well as reduced RNase H activity, for sensitive and efficient full-length cDNA synthesis. Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. The combination of these two engineered enzymes produces enhanced RT-PCR yields and fidelity, as well as amplification of longer templates compared to other one-step RT-PCR kits.

The inclusion of ezDNase (SuperScript IV One-Step RT-PCR System with ezDNase only) for use upstream of the RT step further enhances the one-step RT-PCR workflow through an extremely simplified genomic DNA removal step that ensures the highest accuracy and confidence in one-step RT-PCR results.

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

• SuperScript IV RT Mix (12.5 μL)
• 2X Platinum SuperFi RT-PCR Master Mix (625 μL)
• Nuclease-free water (1.25 mL)

Store at –20°C.

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