TrypLE™ Express Enzyme (1X), no phenol red
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TrypLE™ Express Enzyme (1X), no phenol redGreen features
TrypLE Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO,Read more
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Catalog NumberQuantity
12604013100 mL
12604021500 mL
1260403920 x 100mL
126040541 universaL bag(s)
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Catalog number 12604013
Price (USD)/ 100 mL
27.62
Special Offer
Online exclusive
Ends: 01-Jan-2025
36.83 
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Quantity:
100 mL
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Price (USD)/ 100 mL
27.62
Special Offer
Online exclusive
Ends: 01-Jan-2025
36.83 
Save 9.21 (25%)
Add to cart
TrypLE™ Express Enzyme (1X), no phenol red
Catalog number12604013
Price (USD)/ 100 mL
27.62
Special Offer
Online exclusive
Ends: 01-Jan-2025
36.83 
Save 9.21 (25%)
-
Add to cart
TrypLE Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. TrypLE Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts. We offer a variety of TrypLE formulations for a range of cell culture applications. Compared to trypsin and other dissociation reagents, TrypLE Express is:

• Gentle on cells
• Room temperature-stable
• Easy to use
• Animal origin-free (AOF)
This TrypLE Express is modified as follows:
WithWithout
• EDTA• Phenol Red

The formulation of TrypLE solutions is available, although we keep the enzyme concentration confidential.

Gentle on cells
TrypLE Express' exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.

Room temperature-stable
TrypLE Express remains stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to use
TrypLE Express can be directly substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBS.

Animal origin-free (AOF)
Unlike porcine trypsin, TrypLE Express is free of animal-derived components.

cGMP manufacturing and quality system
For supply chain continuity, we manufacture Gibco TrypLE Express Enzyme (1X), no phenol red at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ChelatorsEDTA
Cell TypeMammalian Cells
ClassificationAnimal Origin-free
Concentration1 X
Culture TypeAdherent Cell Culture
FormLiquid
Product TypeCell Culture Dissociation Reagent
Shelf Life24 Months
SterilitySterile-filtered
Without AdditivesNo Phenol Red
Green FeaturesEnergy efficient, Sustainable packaging
OsmolalityCheck and Record
pH6 to 8
Product LineGibco™
Quantity100 mL
RemarksRoom Temperature Stable
Shipping ConditionRoom Temperature
SourceAnimal origin-free
Tested ForIn vitro Bioassay
Unit Size100 mL
Contents & Storage
Storage conditions: 15°C to 30°C, protect from light
Alternate storage conditions: 2°C to 8°C or -5°C to -20°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture