Gibco™

TrypLE™ Express Enzyme (1X), no phenol red

Catalog number: 12604054
Gibco™

TrypLE™ Express Enzyme (1X), no phenol red

Catalog number: 12604054
Catalog Number
12604054
also known as 12604-054
Unit Size
5 L
Price (USD)
Availability
-
Quantity
Catalog Number
12604013
also known as 12604-013
Unit Size
100 mL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
500 mL
Price (USD)
Availability
-
Quantity
Catalog Number
12604039
also known as 12604-039
Unit Size
20 x 100 mL
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12604054
also known as 12604-054
5 L
-
12604013
also known as 12604-013
100 mL
-
12604021500 mL
-
12604039
also known as 12604-039
20 x 100 mL
-
Product Overview
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TrypLE Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. TrypLE Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts. We offer a variety of TrypLE formulations for a range of cell culture applications. Compared to trypsin and other dissociation reagents, TrypLE Express is:

• Gentle on cells
• Room temperature-stable
• Easy to use
• Animal origin-free (AOF)
This TrypLE Express is modified as follows:
WithWithout
• EDTA• Phenol Red

The formulation of TrypLE solutions is available, although we keep the enzyme concentration confidential.

Gentle on cells
TrypLE Express' exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.

Room temperature-stable
TrypLE Express remains stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to use
TrypLE Express can be directly substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBS.

Animal origin-free (AOF)
Unlike porcine trypsin, TrypLE Express is free of animal-derived components.

cGMP manufacturing and quality system
For supply chain continuity, we manufacture Gibco TrypLE Express Enzyme (1X), no phenol red at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Chelators
EDTA
Cell Type
Mammalian Cells
Classification
Animal Origin-free
Concentration
1 X
Culture Type
Adherent Cell Culture
Form
Liquid
Product Type
Cell Culture Dissociation Reagent
Shelf Life
24 Months
Sterility
Sterile-filtered
Without Additives
No Phenol Red
Green Features
Energy efficient, Sustainable packaging
Osmolality
Check and Record
Product Line
Gibco™
Quantity
1 universaL bag(s)
Remarks
Room Temperature Stable
Shipping Condition
Room Temperature
Source
Animal origin-free
Tested For
In vitro Bioassay

Contents & Storage

Storage conditions: 15°C to 30°C, protect from light
Alternate storage conditions: 2°C to 8°C or -5 to -20°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture

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