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Gibco™

TrypLE™ Express Enzyme (1X), phenol red

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Catalog number: 12605010
Gibco™

TrypLE™ Express Enzyme (1X), phenol red

Green features
Catalog number: 12605010
Catalog Number
12605010
also known as 12605-010
Unit Size
100 mL
Price (USD)
32.57
Your Price:
Availability
***
Quantity
Catalog Number
12605036
also known as 12605-036
Unit Size
20 x 100 mL
Price (USD)
510.00
Your Price:
Availability
***
Quantity
Catalog Number
12605093
also known as 12605-093
Unit Size
5 L
Price (USD)
798.00
Your Price:
Availability
***
Quantity
Catalog Number
12605028
also known as 12605-028
Unit Size
500 mL
Price (USD)
94.25
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12605010
also known as 12605-010
100 mL
32.57
Your Price:
***
12605036
also known as 12605-036
20 x 100 mL
510.00
Your Price:
***
12605093
also known as 12605-093
5 L
798.00
Your Price:
***
12605028
also known as 12605-028
500 mL
94.25
Your Price:
***
Product Overview
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Additional Information
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TrypLE Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A549, primary human keratinocytes, and embryonic stem cells. TrypLE Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts.

We offer a variety of TrypLE formulations for a range of cell culture applications.

Compared to trypsin and other dissociation reagents, Gibco TrypLE Express is:

Gentle on cells

Room-temperature stable
Easy to use
Animal origin-free (AOF)

This TrypLE Express is modified as follows:

With

• Phenol Red

• EDTA

The formulation of TrypLE solutions is available, although we keep the enzyme concentration confidential.

Gentle On Cells
TrypLE Express' exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from enzymes present in some trypsin preparations and in other protease extracts.

Room-Temperature Stable
TrypLE Express is room-temperature stable and ready to use. TrypLE Express remains stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to Use
TrypLE Express can be directly substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBS.

Animal Origin-Free (AOF)
Unlike porcine trypsin, TrypLE Express is free of animal-derived components.

cGMP Manufacturing and Quality System
For supply chain continuity, we manufacture Gibco TrypLE Express at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to the ISO 13485 standard, and are registered with the FDA as medical device manufacturers.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
1X
Product Type
Cell Culture Dissociation Reagent
Sterility
Sterile-filtered
Chelators
EDTA
Enzyme Stability
Room Temperature Stable
pH Range
Check and Record
Osmolality
Check and Record
Shipping Condition
Room Temperature
Tested For
In Vitro Bioassay
Product Line
Gibco™
Form
Liquid
Phenol Red Indicator
Phenol Red
Shelf Life
24 Months
Green Features
Energy efficient, Sustainable packaging
Quantity
100mL
Reagent Type
Trypsin Replacement
Cell Type
Mammalian Cell
With Additives
Phenol Red, EDTA
Culture Type
Adherent Cell Culture
Source
Animal origin-free
Classification
Animal Origin-Free
State at Room Temperature
2°C to 8°C
Solution Type
Trypsin Replacement

Contents & Storage

Storage conditions: 15°C to 30°C, protect from light
Alternate storage conditions: 2°C to 8°C or -5°C to -20°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture

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