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TrypLE™ Express Enzyme (1X), phenol red This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  12605093

 Related applications:

Antibody & Cell Culture Production

| Mammalian Cell Culture

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TrypLE™ Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. TrypLE™ Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts.

We offer a variety of TrypLE™ formulations for a range of cell culture applications.

Compared to trypsin and other dissociation reagents, Gibco® TrypLE™ Express is:

Gentle on cells

Room-temperature stable
Easy to use
Animal origin-free (AOF)

This TrypLE™ Express is modified as follows:


• Phenol Red


The formulation of TrypLE™ solutions is available, although we keep the enzyme concentration confidential.

Gentle On Cells
TrypLE™ Express' exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from enzymes present in some trypsin preparations and in other protease extracts.

Room-Temperature Stable
TrypLE™ Express is room-temperature stable and ready to use. TrypLE™ Express remains stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to Use
TrypLE™ Express can be directly substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Express, avoiding the need for trypsin inhibitors, such as FBS.

Animal Origin-Free (AOF)
Unlike porcine trypsin, TrypLE™ Express is free of animal-derived components.

cGMP Manufacturing and Quality System
For supply chain continuity, we manufacture Gibco® TrypLE™ Express at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to the ISO 13485 standard, and are registered with the FDA as medical device manufacturers.
For Research Use Only. Not for use in diagnostic procedures.


Chelators: EDTA
Phenol Red Indicator: Phenol Red
Classification: Animal Origin-Free
Enzyme Stability: Room Temperature Stable
Concentrated: 1 X
Form: Liquid
Osmolality: Check and Record
Product Line: Gibco®
Product Size: 5 L
Quantity: 1 universal bag(s)
Reagent Type: Trypsin Replacement
Tests Performed: In Vitro Bioassay
pH Range: Check and Record
Green Features: Energy efficient, Sustainable packaging
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 15°C to 30°C, protect from light
Alternate storage conditions: 2°C to 8°C or -5°C to -20°C
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture


Manuals & protocols