Notice: This product will be discontinued on May 1, 2017. For superior performance, we recommend both Phusion High-Fidelity DNA Polymerase and Phusion HF Buffer Pack, detergent-free.

Discoverase™ dHPLC DNA Polymerase

Catalog number: 12607024

Invitrogen™  Related applications: PCR

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Discoverase™ dHPLC DNA Polymerase is a PCR enzyme blend specifically designed for dHPLC and mutation analysis applications on instruments such as the Transgenomic WAVE® System. In accordance with dHPLC instrument manufacturers' recommendations, Discoverase™ contains minimal detergents and will not interfere with the lifespan of dHPLC columns/cartridges downstream (Figure 1).

Discoverase™ dHPLC DNA Polymerase delivers:

• High yield
• High fidelity-8-fold higher fidelity than Taq polymerase alone
• Compatibility with dHPLC instrumentation

Amplification of DNA for downstream use in mutation analysis such as dHPLC
For Research Use Only. Not for use in diagnostic procedures.


Polymerase: Discoverase™ dHPLC DNA Polymerase
Hot Start: No
Fidelity (vs. Taq): 8 X
GC-Rich PCR Performance: Low
Reaction Format: Separate Components
Product Overhang: 3'-A
Reaction Speed: Standard
Final Product Size(s): 5 kb or less
Exonuclease Activity: 3' - 5', 5' - 3'
Product Line: Discoverase™
Product Size: 500 reactions
Shipping Condition: Dry Ice

Contents & storage

Discoverase™ dHPLC DNA Polymerase is an enzyme mix of recombinant Taq DNA Polymerase and the proofreading (3´5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase. Discoverase™ dHPLC DNA Polymerase is supplied with 5X Discoverase™ buffer and 50 mM MgSO4 . Store at -20°C. Guaranteed stable for 6 months when properly stored.


Manuals & protocols