Gibco™

FreeStyle™ CHO Expression Medium

Catalog number: 12651022
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Gibco™

FreeStyle™ CHO Expression Medium

Catalog number: 12651022
Have Questions?
Catalog Number
12651022
Unit Size
6 x 1,000 mL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
1,000 mL
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
126510226 x 1,000 mL
-
126510141,000 mL
-
Product Overview
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FreeStyle™ CHO Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transient transfection of CHO-S cells in suspension without adaptation.

This medium requires supplementation with 8 mM glutamine before use. Gibco™ FreeStyle™ CHO Expression Medium features:

• Formulation without hypoxanthine and thymidine for use with or without dhfr systems
• Ability to support high-density suspension growth and transient transfection of CHO-S cells
• Animal origin–free, protein-free and chemically defined formulation
• Easy use as part of the FreeStyle™ MAX CHO Expression System for protein production
• Scalability in spinner flasks and bioreactors

Formulation without hypoxanthine and thymidine for use with or without dhfr systems
Gibco™ FreeStyle™ CHO Expression Medium is made without hypoxanthine and thymidine for use in dihydrofolate reductase (dhfr) amplification systems. Non-dhfr systems require the addition of HT Supplement before use.

Ability to support high-density suspension growth and transient transfection of CHO-S cells
Gibco™ FreeStyle™ CHO cells in suspension can be cultured directly into Gibco™ FreeStyle™ CHO Expression Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco™ FreeStyle™ CHO Expression Medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations. Cells cultured in Gibco™ FreeStyle™ CHO Expression Medium can be transfected using lipid-based transfection reagents, such as Freestyle™ MAX reagent.

Animal origin–free, protein-free and chemically defined formulation
Gibco™ FreeStyle™ CHO Expression Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest. Gibco™ chemically defined media contain no proteins, hydrolysates, or components of unknown composition.

Easy use as part of the FreeStyle™ MAX CHO Expression System for protein production
The FreeStyle™ MAX CHO Expression System is a breakthrough technology for rapid (see figure) and high-yield mammalian protein production in 30 mL and 1 L batch sizes (see figure). The FreeStyle™ MAX CHO Expression System combines GIBCO™ FreeStyle™ media, FreeStyle™ MAX reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293-F cells in one kit.

Scalability in spinner flasks and bioreactors
Protein production using Gibco™ FreeStyle™ CHO Expression Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement Gibco™ FreeStyle™ CHO Expression Medium with additional Pluronic™ F-68 to avoid sheer stress in the culture.

cGMP manufacturing and quality system
Gibco™ FreeStyle™ CHO Expression Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Expression Medium
Cell Line
FreeStyle™ CHO-S Cells, CHO
Classification
Chemically Defined, Protein-Free, Serum-Free
Culture Type
Suspension Cell Culture
Endotoxin Level
≤0.25 EU/mL, ≤ 0.25 EU/ml
Glutamine
No Glutamine
Phenol Red Indicator
No Phenol Red
Serum Level
Serum-Free
Green Features
Sustainable packaging
Product Line
Freestyle™, Gibco™
Quantity
6 x 1 L
Form
Liquid

Contents & Storage

Storage conditions: 2°C to 8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

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