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Gibco™

DMEM, powder, high glucose, pyruvate

Catalog number: 12800017
Gibco™

DMEM, powder, high glucose, pyruvate

Catalog number: 12800017
Catalog Number
12800017
Unit Size
10 x 1 L
Price (USD)
Price: 70.25
Online price: 65.65
Your price:
Availability
-
Quantity
Catalog Number
12800082
also known as 12800-082
Unit Size
10 L
Price (USD)
Price: 41.46
Online price: 37.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
1280001710 x 1 L
Price: 70.25
Online price: 65.65
Your price:
-
12800082
also known as 12800-082
10 L
Price: 41.46
Online price: 37.65
Your price:
-
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DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This DMEM is modified as follows:
WithWithout
• High Glucose• HEPES
• L-glutamine• Sodium Bicarbonate
• Phenol Red 
• Sodium Pyruvate 


The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Specifications

Cell Line
HeLa, 293, Cos-7, and PC-12
Cell Type
Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Classification
Animal Origin-free
Form
Powder
Product Type
DMEM (Dulbecco's Modified Eagle Medium)
Serum Level
Standard Serum Supplementation
Shelf Life
36 Months From Date of Manufacture
Sterility
Sterile-filtered
With Additives
High Glucose, Glutamine, Phenol Red, Sodium Pyruvate
Without Additives
No HEPES, No Sodium Bicarbonate
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Product Line
Gibco™
Quantity
10 x 1 L
Shipping Condition
Room Temperature

Contents & Storage

Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture

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