For superior PCR performance, upgrade to Invitrogen's new Platinum II Taq Hot-start DNA Polymerase.
Invitrogen™

Platinum™ Hot Start PCR Master Mix (2X)

Catalog number:  13000013

Related applications:

PCR

| Methylation Analysis

| Genotyping & Genomic Profiling

| Sanger Sequencing

Back to top

Description

Invitrogen Platinum Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup. It retains all the features of Platinum Taq DNA Polymerase: specificity, robustness and reliability.

Product features:
• High specificity and increased yields with antibody-mediated hot-start PCR
• Convenient room temperature reaction setup
• Versatile formulation for broad range of amplicons

About Platinum Taq DNA Polymerase
Platinum Taq DNA Polymerase is a recombinant Taq DNA polymerase complexed with proprietary antibodies that inhibit enzyme activity at room temperature, reducing nonspecific target amplification at lower temperatures. The antibodies dissociate during the initial PCR denaturation step and DNA polymerase regains its full activity. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends, and has a 5'→3' exonuclease activity.

Using Platinum Hot Start PCR Master Mix
Convenient Platinum Hot Start PCR Master Mix provides a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup. It is supplied with a separate vial of Platinum GC Enhancer, formulated for specific amplification and improved yields of GC-rich targets. Use Platinum Hot Start PCR Master Mix in PCR applications such as genotyping, gene expression profiling, and next-generation sequencing.

Note: For superior PCR performance, we recommend the next-generation enzyme Platinum II Taq Hot-start DNA Polymerase. Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology. Platinum II Hot-Start PCR Master Mix (2X) contains Platinum II Taq Hot-Start DNA Polymerase in a ready-to-use mixture with Platinum II PCR buffer and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Dye Type: None
Hot Start: Built-In Hot Start
Product Line: Platinum™
Shipping Condition: Dry Ice
Tracking Dye: None
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Medium
No. of Reactions: 200 reactions
Overhang: 3'-A
Polymerase: Platinum™ Taq DNA Polymerase
Reaction Format: SuperMix or Master Mix
Reaction Speed: Standard
Size (Final Product): 5 kb or less

Contents & storage

Each box contains:
• 4 x 1.25 mL Platinum Hot Start PCR 2X Master Mix
• 2 x 1.25 mL Platinum GC Enhancer
• 4 x 1.25 mL water, nuclease-free
Store at -20°C in a non-frost-free freezer.