Bovine Pituitary Extract (BPE)

Catalog Number	Quantity
13028014	25 mg

Catalog number 13028014

Price (USD)

93.50

Quantity:

25 mg

Price (USD)

93.50

The protein concentration of each lot of Bovine Pituitary Extract is determined by the Bradford method. Each vial of BPE contains not less than 25 mg of protein.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell TypeMammalian Cells

Culture TypeMammalian Cell Culture

Quantity25 mg

Shelf Life18 Months

Shipping ConditionDry Ice

FormLiquid

Product TypeBovine Pituitary Extract

pH6 to 8

Unit SizeEach

Contents & Storage

Store in freezer (-5 to -30°C).

Frequently asked questions (FAQs)

Are the Keratinocyte SFM basal medium and supplement kit available separately?

The basal medium is not available for purchase individually, and can only be ordered as part of the Keratinocyte SFM kit (catalog # 17005-042). The supplements are available separately: Bovine Pituitary Extract is catalog # 13028-014 and the EGF is catalog # PHG0311.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture mouse keratinocytes?

The Keratinocyte SFM without calcium chloride (Cat. No. 37010-022) is appropriate for the culture of mouse keratinocytes. The medium should be supplemented with calcium chloride at a final concentration of 0.02 mM.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture human keratinocytes?

There are a variety of options for serum-free culture of human keratinocytes. Keratinocyte SFM (Cat. No. 17005-042) is a widely used kit that includes the basal medium, bovine pituitary extract (BPE) and Epidermal Growth Factor (EGF). If you would like a serum-free, defined culture system, the options are the Defined Keratinocyte SFM kit (Cat. No. 10744-019) or Epilife medium (Cat. No. M-EPI-500-CA) supplemented with EDGS (Cat. No. S-012-5). If you would like an animal origin free defined system, Epilife medium (Cat. No. M-EPI-500-CA) with S7 supplement (Cat. No. S-017-5) is an excellent choice.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (1)

Citations & References

Abstract

Laminin alpha 3 LG4 module induces matrix metalloproteinase-1 through mitogen-activated protein kinase signaling.

Authors:Utani A, Momota Y, Endo H, Kasuya Y, Beck K, Suzuki N, Nomizu M, Shinkai H,

Journal:J Biol Chem

PubMed ID:12826666

'The LG4 module of the laminin alpha 3 chain (alpha 3 LG4), a component of epithelial-specific laminin-5, has cell attachment activity and binds syndecan (Utani, A., Nomizu, M., Matsuura, H., Kato, K., Kobayashi, T., Takeda, U., Aota, S., Nielsen, P. K., and Shinkai, H. (2001) J. Biol. Chem. 276, 28779-28788). ... More