This product is manufactured in our Grand Island, US facility and is intended for sale in North America, Latin America, and the Asia/Pacific region—higher freight charges will apply to orders shipped elsewhere. An equivalent product, manufactured in Paisley, Scotland, is recommended for our customers in Europe, Africa, and the Middle East. Click to view.

DPBS (10X), calcium, magnesium This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 14080055

Gibco™  Related applications: Mammalian Cell Culture

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Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport media or for reagent preparation. We offer a variety of Gibco® DPBS formulations for a range of cell culture applications. Find the right formulation using the media selector tool.

This DPBS is modified as follows:

• Calcium• Phenol Red
• Magnesium

The complete formulation is available.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® DPBS is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® DPBS product made in our Scotland facility (14080-048). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Concentrated forms of Gibco® DPBS require pH adjustment and filtration at the time of preparation (see protocol for details).


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Form: Liquid
Inorganic Salts: Calcium, Magnesium
Concentrated: 10 X
Phenol Red Indicator: No Phenol Red
pH Range: 4.4 - 4.7
Osmolality: 2900 - 3150 mOsm/kg
Product Size: 500 mL
Classification: Animal Origin-Free
Sodium Pyruvate Additive: No Sodium Pyruvate
Green Features: Fewer resources used, Less waste
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8° C.
Shelf life: 24 months from date of manufacture.


Manuals & protocols

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