HBSS, no calcium, no magnesium
HBSS, no calcium, no magnesium
Actual product may vary
Citations & References (2)
Gibco™

HBSS, no calcium, no magnesium

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation,Read more
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Catalog NumberQuantity
14170120100 mL
14170112500 mL
1417016110 x 500 mL
Catalog number 14170120
Price (KRW)
28,000
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Ends: 31-Dec-2025
29,000
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Quantity:
100 mL
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Price (KRW)
28,000
Online offer
Ends: 31-Dec-2025
29,000
Save 1,000 (3%)
Each
Add to cart

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation.

We offer a variety of Gibco™ HBSS formulations for a range of cell culture applications.


This HBSS is modified as follows:
WithWithout
• Phenol Red• Calcium
• Glucose• Magnesium


The complete formulation is available.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Chemical Name or MaterialHanks' Balanced Salt Solution (HBSS)
IncludesNo Sodium Pyruvate
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality265 - 295 mOsm/kg
Recommended StorageStorage conditions: 15-30° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
SterilitySterile-filtered
Concentration1X
For Use With (Application)Mammalian Cell Culture
Physical FormLiquid
Product LineGibco
Quantity100 mL
Solution TypeHank's Balanced Salt
pH6.7 to 7.8
Unit SizeEach

Frequently asked questions (FAQs)

Can I store Hank's Balanced Salt Solution (HBSS) in the refrigerator?

The recommended storage condition for our HBSS products is at 15-30 degrees C. Generally, storage at 4 degrees C will not impact these products. The only concern would be for the 10x HBSS solutions with calcium and magnesium as these salts might precipitate when stored outside the recommended temperature ranges. As long as you don't see any precipitation upon refrigeration, the product will be fine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (2)

Citations & References
Abstract
The Plexin-B1/Rac interaction inhibits PAK activation and enhances Sema4D ligand binding.
Authors: Vikis Haris G; Li Weiquan; Guan Kun-Liang;
Journal:Genes Dev
PubMed ID:11937491
'The small GTPase Rac has been implicated in growth cone guidance mediated by semaphorins and their receptors. Here we demonstrate that plexin-B1, a receptor for Semaphorin4D (Sema4D), and p21-activated kinase (PAK) can compete for the interaction with active Rac and plexin-B1 can inhibit Rac-induced PAK activation. We have also demonstrated ... More
Leukocyte elastase negatively regulates Stromal cell-derived factor-1 (SDF-1)/CXCR4 binding and functions by amino-terminal processing of SDF-1 and CXCR4.
Authors: Valenzuela-Fernández Agustín; Planchenault Thierry; Baleux Françoise; Staropoli Isabelle; Le-Barillec Karine; Leduc Dominique; Delaunay Thierry; Lazarini Françoise; Virelizier Jean-Louis; Chignard Michel; Pidard Dominique; Arenzana-Seisdedos Fernando;
Journal:J Biol Chem
PubMed ID:11867624
Activation of CXCR4 by the CXC chemokine stromal cell-derived factor-1 (SDF-1) requires interaction of the amino-terminal domains of both molecules. We report that proteinases released from either mononucleated blood cells or polymorphonuclear neutrophils degranulated by inflammatory stimuli generate an SDF-1 fragment that is deleted from amino-terminal residues Lys(1)-Pro(2)-Val(3), as characterized ... More