Platinum™ II Taq Hot-Start DNA Polymerase
Inquire about OEM or Commercial Supply version of this product here.
Invitrogen™
Platinum™ II Taq Hot-Start DNA Polymerase
Invitrogen Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.
Have Questions?
Change viewbuttonViewtableView
Catalog NumberNo. of Reactions
14966005500 Reactions
14966001100 Reactions
149660252500 Reactions
149661004 x 2500 Reactions
4 Options
Catalog number 14966005
Price (USD)/ 500 reactions
612.65
Online exclusive
626.00 
Save 13.35 (2%)
-
Add to cart
No. of Reactions:
500 Reactions
Request bulk or custom format
Price (USD)/ 500 reactions
612.65
Online exclusive
626.00 
Save 13.35 (2%)
Add to cart
Platinum™ II Taq Hot-Start DNA Polymerase
Catalog number14966005
Price (USD)/ 500 reactions
612.65
Online exclusive
626.00 
Save 13.35 (2%)
-
Add to cart
Invitrogen Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.

Features of Platinum II Taq Hot-Start DNA Polymerase include:

  • Innovative buffer—enables universal annealing temperature by isostabilizing primer-template duplex structures
  • Engineered Taq DNA polymerase—confers fast cycling and resistance to common inhibitors
  • Platinum hot-start technology—enables superior specificity, sensitivity, and yields; allows for room temperature reaction setup

Platinum II Taq Hot-Start DNA Polymerase is an engineered Taq DNA polymerase that shows increased resistance to reaction inhibitors originating from sample material or DNA purification steps. The polymerase has a higher DNA synthesis rate and delivers PCR results more than two times faster than other Taq DNA polymerases. Proprietary Platinum Taq antibodies block polymerase activity at ambient temperatures and dissociate after the initial denaturation step at 94°C. This automatic 'hot start' provides increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature.

Due to the unique composition of the Platinum II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules. Isostabilizing molecules in the buffer increase primer–template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair. With Platinum II Taq Hot-Start DNA Polymerase, different PCR assays can be cycled together using the same protocol with universal primer annealing temperature and the extension step selected for the longest fragment to be amplified.

Platinum II Taq Hot-Start DNA Polymerase is provided with the optional Platinum GC Enhancer for specific amplification and improved yields of GC-rich targets.

Use Platinum II Taq Hot-Start DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR, in applications like genotyping, high-throughput PCR, or with samples of suboptimal purity.

For increased convenience, we offer Platinum II Hot-Start PCR Master Mix (2X) (Cat. No. 14000012), where Platinum II Taq Hot-Start DNA Polymerase is provided in a ready-to-use mixture with Platinum II PCR buffer and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup. Platinum II Hot-Start Green PCR Master Mix (2X) is also available, which additionally contains a density reagent and two tracking dyes for direct loading of PCR products on gels, further streamlining the PCR workflow from setup to final analysis of the result.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatTube
GC-Rich PCR PerformanceHigh
PolymerasePlatinum II Taq Hot-Start DNA Polymerase
Reaction SpeedFast or Standard
Product TypeHot Start DNA Polymerase
Shipping ConditionDry Ice
For Use With (Application)Hot-start PCR
Concentration2X
Fidelity (vs. Taq)1X
Hot StartBuilt-In Hot Start
No. of Reactions500 Reactions
Overhang3'-A
Reaction FormatSeparate Components
Size (Final Product)5 kb or less
Starting MaterialDNA
Unit Size500 reactions
Contents & Storage
• Platinum II Taq HS DNA Polymerase, 200 μL
• 5X Platinum II PCR Buffer, 4 x 1.25 mL
• Platinum GC Enhancer, 4 x 1.25 mL

Store at -20°C in a non-frost-free freezer.