Decreased metallation and activity in subsets of mutant superoxide dismutases associated with familial amyotrophic lateral sclerosis.
Authors Hayward Lawrence J; Rodriguez Jorge A; Kim Ji W; Tiwari Ashutosh; Goto Joy J; Cabelli Diane E; Valentine Joan Selverstone; Brown Robert H Jr;
JournalJ Biol Chem
PubMed ID11854284
Over 90 different mutations in the gene encoding copper/zinc superoxide dismutase (SOD1) cause approximately 2% of amyotrophic lateral sclerosis (ALS) cases by an unknown mechanism. We engineered 14 different human ALS-related SOD1 mutants and obtained high yields of biologically metallated proteins from an Sf21 insect cell expression system. Both the ... More
Molecular determinants of receptor binding and signaling by the CX3C chemokine fractalkine.
Authors Mizoue L S; Sullivan S K; King D S; Kledal T N; Schwartz T W; Bacon K B; Handel T M;
JournalJ Biol Chem
PubMed ID11432858
Fractalkine/CX3CL1 is a membrane-tethered chemokine that functions as a chemoattractant and adhesion protein by interacting with the receptor CX3CR1. To understand the molecular basis for the interaction, an extensive mutagenesis study of fractalkine's chemokine domain was undertaken. The results reveal a cluster of basic residues (Lys-8, Lys-15, Lys-37, Arg-45, and ... More