This product is manufactured in our Grand Island, US facility and is intended for sale in North America, Latin America, and the Asia/Pacific region—higher freight charges will apply to orders shipped elsewhere. An equivalent product, manufactured in Paisley, Scotland, is recommended for our customers in Europe, Africa, and the Middle East. Click to view.

Trypsin (0.25%), phenol red This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 15050065

Gibco™  Related applications: Mammalian Cell Culture

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Gibco® Trypsin solutions are made from trypsin powder—an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, it is widely used for cell dissociation, during routine cell culture passaging and primary tissue dissociation. We offer a variety of Gibco® Trypsin and animal origin free TrypLE™ that feature:

Quality Testing
Documented Traceability
Dual-site cGMP Manufacturing

This Trypsin is modified as follows:

• Phenol Red• EDTA

The complete formulation is available.

Quality Testing
Gibco® Trypsin solutions are tested for pH, osmolality, sterility and performance. In addition, prior to manufacturing, the raw materials are verified for e-beam irradiation and tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal and viral contamination, as well as multiple activity assays, ash analysis and moisture analysis.

Documented Traceability
We can provide detailed documentation to meet your regulatory needs. Gibco® Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.

cGMP Manufacturing and Quality System
Gibco® Trypsin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco® Trypsin solution made in our Scotland facility (25050-014). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.


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Chelators: No EDTA
Phenol Red Indicator: Phenol Red
Classification: Animal Origin
Form: Liquid
pH Range: 7.2 - 8.0
Osmolality: 280 - 310 mOsm/kg
Concentrated: 1 X
Product Size: 100 mL
Reagent Type: Trypsin
Tests Performed: In Vitro Bioassay
Green Features: Fewer resources used, Less waste
Shipping Condition: Wet Ice

Contents & storage

Storage conditions: -5 to -20°C.
Shelf life: 24 months from date of manufacture.


Manuals & protocols

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