Thermo Scientific™

Pierce™ Protein Coated Plates

Catalog number: 15132
Thermo Scientific™

Pierce™ Protein Coated Plates

Catalog number: 15132
These plates are uniformly and stably coated with one of four popular immunoglobilin-binding proteins (Protein A, Protein G, Protein A/G, or Protein L). They enable antibodies, or pre-established antibody-antigen complexes, to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.
 
Catalog Number
15132
Unit Size
5 plates
Surface Treatment
Protein A
Description
Clear, 8-well Strip
Price (USD)
Price: 271.00
Your Price:
Availability
-
Full specifications
Quantity
Binding PropertyStandard Capacity, Affinity
DescriptionClear, 8-well Strip
Detection MethodColorimetric
Product LinePierce™
ColorClear
Product TypeDetection Plate, Immunoassay, ELISA
MaterialPolystyrene
No. of Wells96
Surface TreatmentProtein A
No. per Pack5 Plates
Plate BlockingSuperBlock™ Buffer
Unit Size5 plates
Catalog Number
Unit Size
5 plates
Surface Treatment
Protein A
Description
Clear, 96-well
Price (USD)
Price: 239.00
Your Price:
Availability
-
Full specifications
Quantity
Binding PropertyStandard Capacity, Affinity
DescriptionClear, 96-well
Detection MethodColorimetric
Product LinePierce™
ColorClear
Product TypeMicroplate
MaterialPolystyrene
No. of Wells96
Surface TreatmentProtein A
No. per Pack5 Plates
Plate BlockingSuperBlock™ Buffer
Unit Size5 plates
Catalog Number
Unit Size
5 plates
Surface Treatment
Protein A
Description
Black, 96-well
Price (USD)
Price: 271.00
Your Price:
Availability
-
Full specifications
Quantity
Binding PropertyStandard Capacity, Affinity
DescriptionBlack, 96-well
Detection MethodFluorescence
Product LinePierce™
ColorBlack
Product TypeDetection Plate, Immunoassay, ELISA
MaterialPolystyrene
No. of Wells96
Surface TreatmentProtein A
No. per Pack5 Plates
Plate BlockingSuperBlock™ Buffer
Unit Size5 plates
Catalog Number
Unit Size
5 plates
Surface Treatment
Protein A
Description
White, 96-well
Price (USD)
Price: 271.00
Your Price:
Availability
-
Full specifications
Quantity
Binding PropertyStandard Capacity, Affinity
DescriptionWhite, 96-well
Detection MethodChemiluminescence
Product LinePierce™
ColorWhite
Product TypeMicroplate
MaterialPolystyrene
No. of Wells96
Surface TreatmentProtein A
No. per Pack5 Plates
Plate BlockingSuperBlock™ Buffer
Unit Size5 plates
Catalog Number
Unit Size
5 plates
Surface Treatment
Protein G
Description
Clear, 8-well Strip
Price (USD)
Price: 294.00
Your Price:
Availability
-
Full specifications
Quantity
Binding PropertyStandard Capacity, Affinity
DescriptionClear, 8-well Strip
Detection MethodColorimetric
Product LinePierce™
ColorClear
Product TypeMicroplate
MaterialPolystyrene
No. of Wells96
Surface TreatmentProtein G
No. per Pack5 Plates
Plate BlockingSuperBlock™ Buffer
Unit Size5 plates
Catalog Number
Unit Size
5 plates
Surface Treatment
Protein G
Description
Clear, 96-well
Price (USD)
Price: 271.00
Your Price:
Availability
-
Full specifications
Quantity
Binding PropertyStandard Capacity, Affinity
DescriptionClear, 96-well
Detection MethodColorimetric
Product LinePierce™
ColorClear
Product TypeDetection Plate, Immunoassay, ELISA
MaterialPolystyrene
No. of Wells96
Surface TreatmentProtein G
No. per Pack5 Plates
Plate BlockingSuperBlock™ Buffer
Unit Size5 plates
Catalog Number
Unit Size
5 plates
Surface Treatment
Protein G
Description
Black, 96-well
Price (USD)
Price: 294.00
Your Price:
Availability
-
Full specifications
Quantity
Binding PropertyStandard Capacity, Affinity
DescriptionBlack, 96-well
Detection MethodFluorescence
Product LinePierce™
ColorBlack
Product TypeMicroplate
MaterialPolystyrene
No. of Wells96
Surface TreatmentProtein G
No. per Pack5 Plates
Plate BlockingSuperBlock™ Buffer
Unit Size5 plates
Catalog Number
Unit Size
5 plates
Surface Treatment
Protein G
Description
White, 96-well
Price (USD)
Price: 294.00
Your Price:
Availability
-
Full specifications
Quantity
Binding PropertyStandard Capacity, Affinity
DescriptionWhite, 96-well
Detection MethodChemiluminescence
Product LinePierce™
ColorWhite
Product TypeMicroplate
MaterialPolystyrene
No. of Wells96
Surface TreatmentProtein G
No. per Pack5 Plates
Plate BlockingSuperBlock™ Buffer
Unit Size5 plates
Catalog Number
Unit Size
5 plates
Surface Treatment
Protein A/G
Description
Clear, 8-well Strip
Price (USD)
Price: 297.00
Your Price:
Availability
-
Full specifications
Quantity
Binding PropertyStandard Capacity, Affinity
DescriptionClear, 8-well Strip
Detection MethodColorimetric
Product LinePierce™
ColorClear
Product TypeDetection Plate, Immunoassay, ELISA
MaterialPolystyrene
No. of Wells96
Surface TreatmentProtein A/G
No. per Pack5 Plates
Plate BlockingSuperBlock™ Buffer
Unit Size5 plates
Catalog Number
Unit Size
5 plates
Surface Treatment
Protein L
Description
Clear, 96-well
Price (USD)
Price: 291.00
Your Price:
Availability
-
Full specifications
Quantity
Binding PropertyStandard Capacity, Affinity
DescriptionClear, 96-well
Detection MethodColorimetric
Product LinePierce™
ColorClear
Product TypeMicroplate
MaterialPolystyrene
No. of Wells96
Surface TreatmentProtein L
No. per Pack5 Plates
Plate BlockingSuperBlock™ Buffer
Unit Size5 plates
Showing 10 of 10
Catalog NumberSpecificationsUnit SizeSurface TreatmentDescriptionPrice (USD)AvailabilityQuantity
15132Full specifications
5 platesProtein AClear, 8-well Strip
Price: 271.00
Your Price:
-
Binding PropertyStandard Capacity, Affinity
DescriptionClear, 8-well Strip
Detection MethodColorimetric
Product LinePierce™
ColorClear
Product TypeDetection Plate, Immunoassay, ELISA
MaterialPolystyrene
No. of Wells96
Surface TreatmentProtein A
No. per Pack5 Plates
Plate BlockingSuperBlock™ Buffer
Unit Size5 plates
15130Full specifications
5 platesProtein AClear, 96-well
Price: 239.00
Your Price:
-
Binding PropertyStandard Capacity, Affinity
DescriptionClear, 96-well
Detection MethodColorimetric
Product LinePierce™
ColorClear
Product TypeMicroplate
MaterialPolystyrene
No. of Wells96
Surface TreatmentProtein A
No. per Pack5 Plates
Plate BlockingSuperBlock™ Buffer
Unit Size5 plates
15155Full specifications
5 platesProtein ABlack, 96-well
Price: 271.00
Your Price:
-
Binding PropertyStandard Capacity, Affinity
DescriptionBlack, 96-well
Detection MethodFluorescence
Product LinePierce™
ColorBlack
Product TypeDetection Plate, Immunoassay, ELISA
MaterialPolystyrene
No. of Wells96
Surface TreatmentProtein A
No. per Pack5 Plates
Plate BlockingSuperBlock™ Buffer
Unit Size5 plates
15154Full specifications
5 platesProtein AWhite, 96-well
Price: 271.00
Your Price:
-
Binding PropertyStandard Capacity, Affinity
DescriptionWhite, 96-well
Detection MethodChemiluminescence
Product LinePierce™
ColorWhite
Product TypeMicroplate
MaterialPolystyrene
No. of Wells96
Surface TreatmentProtein A
No. per Pack5 Plates
Plate BlockingSuperBlock™ Buffer
Unit Size5 plates
15133Full specifications
5 platesProtein GClear, 8-well Strip
Price: 294.00
Your Price:
-
Binding PropertyStandard Capacity, Affinity
DescriptionClear, 8-well Strip
Detection MethodColorimetric
Product LinePierce™
ColorClear
Product TypeMicroplate
MaterialPolystyrene
No. of Wells96
Surface TreatmentProtein G
No. per Pack5 Plates
Plate BlockingSuperBlock™ Buffer
Unit Size5 plates
15131Full specifications
5 platesProtein GClear, 96-well
Price: 271.00
Your Price:
-
Binding PropertyStandard Capacity, Affinity
DescriptionClear, 96-well
Detection MethodColorimetric
Product LinePierce™
ColorClear
Product TypeDetection Plate, Immunoassay, ELISA
MaterialPolystyrene
No. of Wells96
Surface TreatmentProtein G
No. per Pack5 Plates
Plate BlockingSuperBlock™ Buffer
Unit Size5 plates
15157Full specifications
5 platesProtein GBlack, 96-well
Price: 294.00
Your Price:
-
Binding PropertyStandard Capacity, Affinity
DescriptionBlack, 96-well
Detection MethodFluorescence
Product LinePierce™
ColorBlack
Product TypeMicroplate
MaterialPolystyrene
No. of Wells96
Surface TreatmentProtein G
No. per Pack5 Plates
Plate BlockingSuperBlock™ Buffer
Unit Size5 plates
15156Full specifications
5 platesProtein GWhite, 96-well
Price: 294.00
Your Price:
-
Binding PropertyStandard Capacity, Affinity
DescriptionWhite, 96-well
Detection MethodChemiluminescence
Product LinePierce™
ColorWhite
Product TypeMicroplate
MaterialPolystyrene
No. of Wells96
Surface TreatmentProtein G
No. per Pack5 Plates
Plate BlockingSuperBlock™ Buffer
Unit Size5 plates
15138Full specifications
5 platesProtein A/GClear, 8-well Strip
Price: 297.00
Your Price:
-
Binding PropertyStandard Capacity, Affinity
DescriptionClear, 8-well Strip
Detection MethodColorimetric
Product LinePierce™
ColorClear
Product TypeDetection Plate, Immunoassay, ELISA
MaterialPolystyrene
No. of Wells96
Surface TreatmentProtein A/G
No. per Pack5 Plates
Plate BlockingSuperBlock™ Buffer
Unit Size5 plates
15190Full specifications
5 platesProtein LClear, 96-well
Price: 291.00
Your Price:
-
Binding PropertyStandard Capacity, Affinity
DescriptionClear, 96-well
Detection MethodColorimetric
Product LinePierce™
ColorClear
Product TypeMicroplate
MaterialPolystyrene
No. of Wells96
Surface TreatmentProtein L
No. per Pack5 Plates
Plate BlockingSuperBlock™ Buffer
Unit Size5 plates
Showing 10 of 10

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Thermo Scientific Pierce Protein Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

Highlights
• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)

Protein A/G Coated Plates
• Fusion protein containing four antibody-binding sites from Protein A and two from Protein G
• Binds to most IgG species because it combines the specificities of Protein A and Protein G
• Binds to Fc region of antibodies, ensuring optimal orientation
• Binds well using a wide range of pH

Protein A Coated Plates
• Binds strongly to IgG from human, rabbit, guinea pig, pig, dog, and rhesus monkey
• Binds strongly to mouse IgG2a, IgG2b and IgG3
• Binds to Fc region of antibodies for optimal orientation

Protein G Coated Plates
• Binds strongly to IgG from many species including human, mouse, rabbit, sheep and goat
• Binds only to IgG—no cross-reactivity with other antibody classes
• Binds to Fc region of antibodies for optimal orientation

Protein L Coated Plates
• Binds to all classes of immunoglobulins (IgG, IgM, IgA, IgE and IgD)
• Binds to the VL region of kappa light chains (human I, III, IV and Mouse I) without interfering with antigen-binding sites
• Binds to Fab fragments and ScFv
• Does not bind bovine, goat or sheep immunoglobulin
• Binds weakly to rabbit immunoglobulin
• Pre-blocked to reduce nonspecific binding

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Note: Plates are activated to 1 µL and supplied pre-blocked with Thermo Scientific SuperBlock Blocking Buffer. Use these plates for single-antibody assays only. In multiple antibody assays, such as sandwich ELISAs, the first antibody cannot block all binding sites and subsequent antibodies will bind to the plate, resulting in false positives.

Upon receipt store plates at 4°C in unopened pouches. Place opened, unused plates in a resealable bag and store desiccated at 4°C. Plates are shipped at ambient temperature.