Pierce™ Protein A Coated Plates, Clear, 8-Well Strip

Catalog number: 15132

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Thermo Scientific Pierce Protein A Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

These plates are uniformly and stably coated with Protein A, and enable antibodies or pre-established antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Features of the plates:

• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)

Protein A Coated Microplates:
• Binds strongly to IgG from human, rabbit, guinea pig, pig, dog and rhesus monkey
• Binds strongly to mouse IgG2a, IgG2b and IgG3
• Binds to Fc region of antibodies for optimal orientation

Related Products
Pierce™ Protein A Coated Plates, Clear, 96-Well
Pierce™ Protein A Coated Plates, White, 96-Well
Pierce™ Protein A Coated Plates, Black, 96-Well
For Research Use Only. Not for use in diagnostic procedures.


Color: Clear
Format: 96-well strip plate
Plate Type: Detection Plate, ELISA, Immunoassay
Product Size: 5 plates
Plate Blocking: SuperBlock™ Buffer
Surface Coating: Protein A
Binding Property: Affinity, Standard Capacity
Detection Method: Colorimetric

Contents & storage

Note: Plates are activated to 1µL and supplied pre-blocked with Thermo Scientific SuperBlock Blocking Buffer. Use these plates for single-antibody assays only. In multiple antibody assays, such as sandwich ELISAs, the first antibody cannot block all binding sites and subsequent antibodies will bind to the plate, resulting in false positives.

Upon receipt store plates at 4°C in unopened pouches. Place opened, unused plates in a resealable bag and store desiccated at 4°C. Plates are shipped at ambient temperature.


Manuals & protocols