Thermo Scientific™

Pierce™ Copper Coated High Capacity Plates, Black, 96-Well

Catalog number:  15148

 Related applications:

Protein Assays and Analysis

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Thermo Scientific Pierce Copper Coated High Binding Capacity (HBC) Plates use an exclusive coating process to increase the amount of histidine-tagged protein that will bind to the plate surface.

These copper coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metals such as copper. Pierce Copper Coated Plates have high binding capacity because they are coating using an exclusive process that increases the amount of histidine-tagged protein that will bind to the plate surface. Although copper is less discriminating in its binding specificity than Nickel, the higher binding capacity of these plates is ideal for high throughput screening applications that need improved sensitivity and greater dynamic range. The copper-coated plates are available as clear, white or black plates that can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.

Features of Copper Coated High Binding Capacity Plates:

• Quantitate previously undetectable histidine-tagged proteins
• Wider dynamic range with four-fold greater capacity than regular nickel chelate-coated plate
• Copper chelate provides greater binding capacity than nickel
• The detection limit is 0.1 pg of polyhistidine tagged fusion protein per well
• Pre-blocked for the ultimate convenience

Note: Copper coated plates are for use with purified His-tagged proteins. Typical cell lysates contain non-his-tagged proteins that may bind to copper, which can reduce binding capacity and signal-to-noise ratios. For cell lysates, use Thermo Scientific Pierce Nickel Coated Plates.

Related Products
Pierce™ Copper Coated High Capacity Plates, Clear, 96-Well
Pierce™ Copper Coated High Capacity Plates, Clear, 8-Well Strip
Pierce™ Copper Coated High Capacity Plates, White, 96-Well
For Research Use Only. Not for use in diagnostic procedures.


Binding Property: Affinity, High Capacity
Color: Black
Detection Method: Fluorescent
Format: 96-well plate
Plate Blocking: Bovine Serum Albumin (BSA)
Plate Type: Detection Plate, ELISA, Immunoassay
Product Line: Pierce™
Product Size: 5 plates
Surface Coating: Divalent Copper, Metal Chelate

Contents & storage

Store at 4°C in unopened pouches. Once opened, store unused plates in a resealable bag sith desiccant and store at 4°C.


Manuals & protocols