Thermo Scientific™

Pierce™ Nickel Coated Plates

Catalog number: 15242
Thermo Scientific™

Pierce™ Nickel Coated Plates

Catalog number: 15242
These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking. The clear, white or black plates can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.
 
Catalog Number
15242
Unit Size
5 plates
Description
White, 96-well
Detection Method
Chemiluminescence
Price (USD)
Price: 270.00
Your Price:
Availability
***
Full specifications
Quantity
Binding PropertyStandard Capacity, Affinity
DescriptionWhite, 96-well
Detection MethodChemiluminescence
Packaging5 Plates
Product LinePierce™
ColorWhite
Product TypeMicroplate
MaterialPolystyrene
Volume (Metric) Well200 μL
No. of Wells96
Surface TreatmentDivalent Nickel, Metal Chelate
Plate BlockingBlocker™ BSA Buffer
Unit Size5 plates
Catalog Number
Unit Size
5 plates
Description
Black, 96-well
Detection Method
Fluorescence
Price (USD)
Price: 270.00
Your Price:
Availability
***
Full specifications
Quantity
Binding PropertyStandard Capacity, Affinity
DescriptionBlack, 96-well
Detection MethodFluorescence
Packaging5 Plates
Product LinePierce™
ColorBlack
Product TypeMicroplate
MaterialPolystyrene
Volume (Metric) Well200 μL
No. of Wells96
Surface TreatmentDivalent Nickel, Metal Chelate
Plate BlockingBlocker™ BSA Buffer
Unit Size5 plates
Catalog Number
Unit Size
5 plates
Description
Clear, 8-well Strip
Detection Method
Colorimetric
Price (USD)
Price: 270.00
Your Price:
Availability
***
Full specifications
Quantity
Binding PropertyStandard Capacity, Affinity
DescriptionClear, 8-well Strip
Detection MethodColorimetric
Packaging5 Plates
Product LinePierce™
ColorClear
Product TypeMicroplate
MaterialPolystyrene
Volume (Metric) Well200 μL
No. of Wells96
Surface TreatmentDivalent Nickel, Metal Chelate
Plate BlockingBlocker™ BSA Buffer
Unit Size5 plates
Catalog Number
Unit Size
5 plates
Description
Clear, 96-well
Detection Method
Colorimetric
Price (USD)
Price: 264.00
Your Price:
Availability
***
Full specifications
Quantity
Binding PropertyStandard Capacity, Affinity
DescriptionClear, 96-well
Detection MethodColorimetric
Packaging5 Plates
Product LinePierce™
ColorClear
Product TypeMicroplate
MaterialPolystyrene
Volume (Metric) Well200 μL
No. of Wells96
Surface TreatmentDivalent Nickel, Metal Chelate
Plate BlockingBlocker™ BSA Buffer
Unit Size5 plates
Showing 4 of 4
Catalog NumberSpecificationsUnit SizeDescriptionDetection MethodPrice (USD)AvailabilityQuantity
15242Full specifications
5 platesWhite, 96-wellChemiluminescence
Price: 270.00
Your Price:
***
Binding PropertyStandard Capacity, Affinity
DescriptionWhite, 96-well
Detection MethodChemiluminescence
Packaging5 Plates
Product LinePierce™
ColorWhite
Product TypeMicroplate
MaterialPolystyrene
Volume (Metric) Well200 μL
No. of Wells96
Surface TreatmentDivalent Nickel, Metal Chelate
Plate BlockingBlocker™ BSA Buffer
Unit Size5 plates
15342Full specifications
5 platesBlack, 96-wellFluorescence
Price: 270.00
Your Price:
***
Binding PropertyStandard Capacity, Affinity
DescriptionBlack, 96-well
Detection MethodFluorescence
Packaging5 Plates
Product LinePierce™
ColorBlack
Product TypeMicroplate
MaterialPolystyrene
Volume (Metric) Well200 μL
No. of Wells96
Surface TreatmentDivalent Nickel, Metal Chelate
Plate BlockingBlocker™ BSA Buffer
Unit Size5 plates
15142Full specifications
5 platesClear, 8-well StripColorimetric
Price: 270.00
Your Price:
***
Binding PropertyStandard Capacity, Affinity
DescriptionClear, 8-well Strip
Detection MethodColorimetric
Packaging5 Plates
Product LinePierce™
ColorClear
Product TypeMicroplate
MaterialPolystyrene
Volume (Metric) Well200 μL
No. of Wells96
Surface TreatmentDivalent Nickel, Metal Chelate
Plate BlockingBlocker™ BSA Buffer
Unit Size5 plates
15442Full specifications
5 platesClear, 96-wellColorimetric
Price: 264.00
Your Price:
***
Binding PropertyStandard Capacity, Affinity
DescriptionClear, 96-well
Detection MethodColorimetric
Packaging5 Plates
Product LinePierce™
ColorClear
Product TypeMicroplate
MaterialPolystyrene
Volume (Metric) Well200 μL
No. of Wells96
Surface TreatmentDivalent Nickel, Metal Chelate
Plate BlockingBlocker™ BSA Buffer
Unit Size5 plates
Showing 4 of 4
Thermo Scientific Pierce Nickel Coated White 96-Well Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins. These white plates can be used with chemiluminescent detection methods.

Features of Pierce Nickel Coated Plates include:
• Ni2+ activated surface enables metal-chelate binding of His-tagged proteins
• Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene
• Better binding for sensitive assays compared to other commercially available nickel-activated plates
• Detection limit: 1 ng of polyhistidine fusion protein
• Binding capacity: approx. 9 pmol His-tagged protein (27 kDa) per well
• Activation level: 200 µL

These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking.

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C.