Thermo Scientific™

Pierce™ Nickel Coated Plates, Black, 96-Well

Catalog number:  15342

 Related applications:

Protein Assays and Analysis

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Thermo Scientific Pierce Nickel Coated Black 96-Well Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins. These black plates can be used with fluorescent detection methods.

Features of Pierce Nickel Coated Plates:

• Ni2+ activated surface enables metal-chelate binding of His-tagged proteins
• Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene
• Better binding for sensitive assays compared to other commercially available nickel-activated plates
• Detection limit: 1ng of polyhistidine fusion protein
• Binding capacity: approx. 9 pmol His-tagged protein (27kDa) per well
• Activation level: 200 µL

These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking.

Related Products
Pierce™ Nickel Coated Plates, Clear, 96-Well
Pierce™ Nickel Coated Plates, Clear, 8-Well Strip
Pierce™ Nickel Coated Plates, White, 96-Well
For Research Use Only. Not for use in diagnostic procedures.


Binding Property: Affinity, Standard Capacity
Color: Black
Detection Method: Fluorescent
Format: 96-well plate
Plate Blocking: Bovine Serum Albumin (BSA)
Plate Type: Detection Plate, ELISA, Immunoassay
Product Line: Pierce™
Product Size: 5 plates
Surface Coating: Divalent Nickel, Metal Chelate

Contents & storage

Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C.


Manuals & protocols