Why do solutions containing SDS form precipitates?
SDS is a detergent used to denature proteins leading to disruption of cell membranes and dissociation of nucleic acid-protein complexes in DNA and RNA extractions. It tends to come out of solution at concentrations of 10% or greater, especially at low temperatures or in high-salt buffers. SDS can be resolubilized by slowly heating the solution to 65°C with slight agitation.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.