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0.1-2 Kb RNA Ladder - FAQs

View additional product information for 0.1-2 Kb RNA Ladder - FAQs (15623100)

2 product FAQs found

Why are my RNA bands not sharp?

(1) RNA was not completely denatured. Electrophorese RNA under denaturing conditions. Use urea, formamide or formaldehyde gels, or glyoxal-treated RNA.

(2) For glyoxal-treated RNA, a buffer gradient formed during electrophoresis. Recirculate buffer during electrophoresis to prevent gradient formation.

Why are the RNA bands disappearing when looking and photographing a gel on a UV box?

RNA was exposed to UV light for extended periods of time. Minimize exposure to UV light. Stain and destain gels in the dark and photograph the gel immediately.