Zitierungen und Referenzen (8)

Invitrogen™

UltraPure™ Lachssperma-DNA-Lösung

Katalognummer	Menge
15632011	5 x 1 ml

Katalognummer 15632011

Preis (EUR)

168,65

Exklusiv online

183,00

Ersparnis 14,35 (8%)

Menge:

5 x 1 ml

Großbestellung oder individuelle Größe anfordern

Preis (EUR)

168,65

Exklusiv online

183,00

Ersparnis 14,35 (8%)

UltraPure™ Lachssperma-DNA-Lösung ist eine gebrauchsfertige, gescherte DNA-Lösung, die für die direkte Herstellung von Prähybridisierungs- und Hybridisierunglösungen verwendet wird. Diese DNA-Lösung wird aus hochreiner, mit Phenol/Chloroform extrahierter DNA und DNase-freiem, RNase-freiem (nach DEPC-Behandlung), destilliertem, entionisiertem Wasser hergestellt. Die DNA ist auf eine durchschnittliche Größe von ≤2.000 bp abgeschert. Die Konzentration wird auf 10 mg/ml eingestellt.

Anwendungen
Solche Lösungen werden verwendet, um die unspezifische Befestigung der Sonde an der Oberfläche einer Membran zu blockieren.

Leistungs- und Qualitätsprüfung
Konzentration, Größenüberprüfung, Viskosität und ultraviolette Absorption (A₂₆₀/A₂₈₀) werden analytisch getestet.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

Zur Verwendung mit (Anwendung)Chromatinbiologie, Nukleinsäure-Gelelektrophorese, Blotting

HauptbedienfelderUnspezifische Hybridisierung blockieren

ProduktlinieUltraPure

ProdukttypLachsspermien-DNA-Lösung

Menge5 x 1 ml

ReagenztypLachssperma-DNA

VersandbedingungTrockeneis

Unit SizeEach

Inhalt und Lagerung

Inhalt: 5 Röhrchen Lachssperma-DNA-Lösung (je 1 ml, 10 mg/ml)

UltraPure™ Lachssperma-DNA ist 1 Jahr lang bei –20 °C stabil. Das Produkt ist für 6 Monate ab Kaufdatum garantiert, sofern in der Produktdokumentation nichts anderes angegeben ist.

Häufig gestellte Fragen (FAQ)

What species of salmon is Ultrapure Salmon Sperm DNA derived from?

The species of salmon is Oncerhyncus keta.

Is Ultrapure Salmon Sperm DNA double stranded? How can I make it single stranded?

Ultrapure Salmon Sperm DNA is double stranded. It can be made single stranded by heating to 95 degrees C and then cooling rapidly on ice. Cooling is usually done with the DNA diluted in a hybridization solution which keeps it single stranded because of the large dilution volume.

What concentration should the UltraPure Salmon Sperm DNA Solution (Cat. No. 15632011) be used at?

Carrier DNA is typically used at a concentration of 100 µg/mL in both the prehybridization and hybridization solutions.

Zitierungen und Referenzen (8)

Zitierungen und Referenzen

Abstract

The human organic cation transporter (hOCT2) recognizes the degree of substrate ionization.

Authors: Barendt Wendy M; Wright Stephen H;

Journal:J Biol Chem

PubMed ID:11953440

'The organic cation transporter, OCT2, plays a role in renal secretion of a broad array of weak bases. To determine whether the degree of ionization of these compounds plays a role in their interaction with OCT2, we examined the influence of external pH values on the activity of the human ... More

The role of polymer nanolayer architecture on the separation performance of anion-exchange membrane adsorbers: part II. DNA and virus separations.

Authors:Bhut BV, Weaver J, Carter AR, Wickramasinghe SR, Husson SM,

Journal:Biotechnol Bioeng

PubMed ID:21618476

'The surface-initiated polymerization protocol developed in part I was used to prepare strong anion-exchange membranes with variable polymer chain graft densities and degrees of polymerization for DNA and virus particle separations. A focus of part II was to evaluate the role of polymer nanolayer architecture on DNA and virus binding. ... More

Spectrophotometric analysis of nucleic acids: oxygenation-dependent hyperchromism of DNA.

Authors:Doshi R, Day PJ, Carampin P, Blanch E, Stratford IJ, Tirelli N,

Journal:Anal Bioanal Chem

PubMed ID:20169336

'The absorbance at 260 nm (A(260)) is ubiquitously used for nucleic acid quantification. We show that following oxygenation, DNA solutions experience alterations in both spectral properties (hyperchromism in the UV region, lambda(max) 260 nm) and DNA conformation. The spectral changes caused by oxygen-DNA complexation are stable for at least several ... More

A quantitative real-time polymerase chain reaction assay for the seagrass pathogen Labyrinthula zosterae.

Authors:Bergmann N, Fricke B, Schmidt MC, Tams V, Beining K, Schwitte H, Boettcher AA, Martin DL, Bockelmann AC, Reusch TB, Rauch G,

Journal:Mol Ecol Resour

PubMed ID:21777400

'The protist Labyrinthula zosterae (Phylum Bigyra, sensu Tsui et al. 2009) has been identified as a causative agent of wasting disease in eelgrass (Zostera marina), of which the most intense outbreak led to the destruction of 90% of eelgrass beds in eastern North America and western Europe in the 1930s. ... More

Noncoding DNA in lipofection of HeLa cells-a few insights.

Authors:Symens N, Rejman J, Lucas B, Demeester J, De Smedt SC, Remaut K,

Journal:Mol Pharm

PubMed ID:23421924

'In cationic carrier-mediated gene delivery, the disproportional relationship between the quantity of delivered DNA and the amount of encoded protein produced is a well-known phenomenon. The numerous intracellular barriers which need to be overcome by pDNA to reach the nucleoplasm play a major role in it. In contrast to what ... More

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