Thermo Scientific™

Acclaim™ PepMap™ 100 C18 HPLC Columns

Catalog number: 160321
Thermo Scientific™

Acclaim™ PepMap™ 100 C18 HPLC Columns

Catalog number: 160321

Feature high loading capacity for exceptional suitability in the analysis of low abundant peptides in complex proteomics samples with Thermo Scientific™ PepMap™ 100 C18 LC Columns. PepMap LC columns offer lengths targeting fast or high resolution peptide separation.

 
Catalog Number
160321
Unit Size
Each
Particle Size
3 μm
Diameter (Metric)
0.075 mm
Length (Metric)
150 mm
Type
Analytical Column
Price (USD)
798.00
Your Price:
Availability
***
Full specifications
Quantity
Column FormatAnalytical Column
Diameter (Metric)0.075 mm
Flow Rate40 nL/min
For Use WithLC/MS, ESI-MS, MALDI-MS
Length (Metric)150 mm
Max. Pressure7250 psi (500 bar)
Particle Size3 μm
pH2 to 8
Pore Size100 Å
Surface Area450 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load15%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
MaterialFused Silica
TypeAnalytical Column
Unit SizeEach
Catalog Number
Unit Size
Each
Particle Size
3 μm
Diameter (Metric)
0.075 mm
Length (Metric)
50 mm
Type
Analytical Column, NanoViper
Price (USD)
965.00
Your Price:
Availability
***
Full specifications
Quantity
Column FormatAnalytical Column, NanoViper
Diameter (Metric)0.075 mm
Flow Rate200 nL/min
For Use WithLC/MS, ESI-MS, MALDI-MS
Length (Metric)50 mm
Max. Pressure7250 psi (500 bar)
Particle Size3 μm
pH2 to 8
Pore Size100 Å
Surface Area450 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load15%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
MaterialFused Silica
TypeAnalytical Column, NanoViper
Unit SizeEach
Catalog Number
Unit Size
Each
Particle Size
3 μm
Diameter (Metric)
0.075 mm
Length (Metric)
150 mm
Type
Analytical Column, NanoViper
Price (USD)
965.00
Your Price:
Availability
***
Full specifications
Quantity
Column FormatAnalytical Column, NanoViper
Diameter (Metric)0.075 mm
Flow Rate200 nL/min
For Use WithLC/MS, ESI-MS, MALDI-MS
Length (Metric)150 mm
Max. Pressure7250 psi (500 bar)
Particle Size3 μm
pH2 to 8
Pore Size100 Å
Surface Area450 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load15%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
MaterialFused Silica
TypeAnalytical Column, NanoViper
Unit SizeEach
Catalog Number
Unit Size
Each
Particle Size
3 μm
Diameter (Metric)
0.075 mm
Length (Metric)
250 mm
Type
Analytical Column, NanoViper
Price (USD)
1,052.00
Your Price:
Availability
***
Full specifications
Quantity
Column FormatAnalytical Column, NanoViper
Diameter (Metric)0.075 mm
Flow Rate200 nL/min
For Use WithLC/MS, ESI-MS, MALDI-MS
Length (Metric)250 mm
Max. Pressure7250 psi (500 bar)
Particle Size3 μm
pH2 to 8
Pore Size100 Å
Surface Area450 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load15%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
MaterialFused Silica
TypeAnalytical Column, NanoViper
Unit SizeEach
Catalog Number
Unit Size
Each
Particle Size
3 μm
Diameter (Metric)
0.075 mm
Length (Metric)
500 mm
Type
Analytical Column, NanoViper
Price (USD)
1,196.00
Your Price:
Availability
***
Full specifications
Quantity
Column FormatAnalytical Column, NanoViper
Diameter (Metric)0.075 mm
Flow Rate200 nL/min
For Use WithLC/MS, ESI-MS, MALDI-MS
Length (Metric)500 mm
Max. Pressure7250 psi (500 bar)
Particle Size3 μm
pH2 to 8
Pore Size100 Å
Surface Area450 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load15%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
MaterialFused Silica
TypeAnalytical Column, NanoViper
Unit SizeEach
Catalog Number
Unit Size
Each
Particle Size
3 μm
Diameter (Metric)
1 mm
Length (Metric)
150 mm
Type
Analytical Column, NanoViper
Price (USD)
720.00
Your Price:
Availability
***
Full specifications
Quantity
Column FormatAnalytical Column, NanoViper
Diameter (Metric)1 mm
Flow Rate200 nL/min
For Use WithLC/MS, ESI-MS, MALDI-MS
Length (Metric)150 mm
Max. Pressure7250 psi (500 bar)
Particle Size3 μm
pH2 to 8
Pore Size100 Å
Surface Area450 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load15%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
MaterialFused Silica
TypeAnalytical Column, NanoViper
Unit SizeEach
Catalog Number
Unit Size
Pack of 5
Particle Size
5 μm
Diameter (Metric)
1 mm
Length (Metric)
5 mm
Type
Trap Cartridge
Price (USD)
454.00
Your Price:
Availability
***
Full specifications
Quantity
Column FormatTrap Cartridge
Diameter (Metric)1 mm
For Use WithUltiMate 3000 Proteomics MDLC System
Length (Metric)5 mm
Max. Pressure7250 psi (500 bar)
Particle Size5 μm
pH2 to 8
Pore Size100 Å
Surface Area450 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load15%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
MaterialStainless Steel
TypeTrap Cartridge
Unit SizePack of 5
Catalog Number
Unit Size
Pack of 5
Particle Size
5 μm
Diameter (Metric)
0.3 mm
Length (Metric)
5 mm
Type
Trap Cartridge
Price (USD)
454.00
Your Price:
Availability
***
Full specifications
Quantity
Column FormatTrap Cartridge
Diameter (Metric)0.3 mm
For Use WithUltiMate 3000 Proteomics MDLC System
Length (Metric)5 mm
Max. Pressure7250 psi (500 bar)
Particle Size5 μm
pH2 to 8
Pore Size100 Å
Surface Area450 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load15%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
MaterialStainless Steel
TypeTrap Cartridge
Unit SizePack of 5
Catalog Number
Unit Size
Each
Particle Size
5 μm
Diameter (Metric)
1 mm
Length (Metric)
15 mm
Type
Trap Cartridge
Price (USD)
Availability
***
Full specifications
Quantity
-
Column FormatTrap Cartridge
Diameter (Metric)1 mm
For Use WithUltiMate 3000 Proteomics MDLC System
Length (Metric)15 mm
Max. Pressure7250 psi (500 bar)
Particle Size5 μm
pH2 to 8
Pore Size100 Å
Surface Area450 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load15%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
MaterialStainless Steel
TypeTrap Cartridge
Unit SizeEach
Catalog Number
Unit Size
Pack of 2
Particle Size
5 μm
Diameter (Metric)
0.1 mm
Length (Metric)
10 mm
Type
Trap Column
Price (USD)
305.00
Your Price:
Availability
***
Full specifications
Quantity
Column FormatTrap Column
Diameter (Metric)0.1 mm
Length (Metric)10 mm
Max. Pressure7250 psi (500 bar)
Particle Size5 μm
pH2 to 8
Pore Size100 Å
Surface Area450 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load15%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
MaterialFused Silica
TypeTrap Column
Unit SizePack of 2
Showing 10 of 34Show more
Catalog NumberSpecificationsUnit SizeParticle SizeDiameter (Metric)Length (Metric)TypePrice (USD)AvailabilityQuantity
160321Full specifications
Each3 μm0.075 mm150 mmAnalytical Column
798.00
Your Price:
***
Column FormatAnalytical Column
Diameter (Metric)0.075 mm
Flow Rate40 nL/min
For Use WithLC/MS, ESI-MS, MALDI-MS
Length (Metric)150 mm
Max. Pressure7250 psi (500 bar)
Particle Size3 μm
pH2 to 8
Pore Size100 Å
Surface Area450 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load15%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
MaterialFused Silica
TypeAnalytical Column
Unit SizeEach
164567Full specifications
Each3 μm0.075 mm50 mmAnalytical Column, NanoViper
965.00
Your Price:
***
Column FormatAnalytical Column, NanoViper
Diameter (Metric)0.075 mm
Flow Rate200 nL/min
For Use WithLC/MS, ESI-MS, MALDI-MS
Length (Metric)50 mm
Max. Pressure7250 psi (500 bar)
Particle Size3 μm
pH2 to 8
Pore Size100 Å
Surface Area450 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load15%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
MaterialFused Silica
TypeAnalytical Column, NanoViper
Unit SizeEach
164568Full specifications
Each3 μm0.075 mm150 mmAnalytical Column, NanoViper
965.00
Your Price:
***
Column FormatAnalytical Column, NanoViper
Diameter (Metric)0.075 mm
Flow Rate200 nL/min
For Use WithLC/MS, ESI-MS, MALDI-MS
Length (Metric)150 mm
Max. Pressure7250 psi (500 bar)
Particle Size3 μm
pH2 to 8
Pore Size100 Å
Surface Area450 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load15%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
MaterialFused Silica
TypeAnalytical Column, NanoViper
Unit SizeEach
164569Full specifications
Each3 μm0.075 mm250 mmAnalytical Column, NanoViper
1,052.00
Your Price:
***
Column FormatAnalytical Column, NanoViper
Diameter (Metric)0.075 mm
Flow Rate200 nL/min
For Use WithLC/MS, ESI-MS, MALDI-MS
Length (Metric)250 mm
Max. Pressure7250 psi (500 bar)
Particle Size3 μm
pH2 to 8
Pore Size100 Å
Surface Area450 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load15%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
MaterialFused Silica
TypeAnalytical Column, NanoViper
Unit SizeEach
164570Full specifications
Each3 μm0.075 mm500 mmAnalytical Column, NanoViper
1,196.00
Your Price:
***
Column FormatAnalytical Column, NanoViper
Diameter (Metric)0.075 mm
Flow Rate200 nL/min
For Use WithLC/MS, ESI-MS, MALDI-MS
Length (Metric)500 mm
Max. Pressure7250 psi (500 bar)
Particle Size3 μm
pH2 to 8
Pore Size100 Å
Surface Area450 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load15%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
MaterialFused Silica
TypeAnalytical Column, NanoViper
Unit SizeEach
164572Full specifications
Each3 μm1 mm150 mmAnalytical Column, NanoViper
720.00
Your Price:
***
Column FormatAnalytical Column, NanoViper
Diameter (Metric)1 mm
Flow Rate200 nL/min
For Use WithLC/MS, ESI-MS, MALDI-MS
Length (Metric)150 mm
Max. Pressure7250 psi (500 bar)
Particle Size3 μm
pH2 to 8
Pore Size100 Å
Surface Area450 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load15%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
MaterialFused Silica
TypeAnalytical Column, NanoViper
Unit SizeEach
160434Full specifications
Pack of 55 μm1 mm5 mmTrap Cartridge
454.00
Your Price:
***
Column FormatTrap Cartridge
Diameter (Metric)1 mm
For Use WithUltiMate 3000 Proteomics MDLC System
Length (Metric)5 mm
Max. Pressure7250 psi (500 bar)
Particle Size5 μm
pH2 to 8
Pore Size100 Å
Surface Area450 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load15%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
MaterialStainless Steel
TypeTrap Cartridge
Unit SizePack of 5
160454Full specifications
Pack of 55 μm0.3 mm5 mmTrap Cartridge
454.00
Your Price:
***
Column FormatTrap Cartridge
Diameter (Metric)0.3 mm
For Use WithUltiMate 3000 Proteomics MDLC System
Length (Metric)5 mm
Max. Pressure7250 psi (500 bar)
Particle Size5 μm
pH2 to 8
Pore Size100 Å
Surface Area450 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load15%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
MaterialStainless Steel
TypeTrap Cartridge
Unit SizePack of 5
160438Full specifications
Each5 μm1 mm15 mmTrap CartridgeContact Us ›***-
Column FormatTrap Cartridge
Diameter (Metric)1 mm
For Use WithUltiMate 3000 Proteomics MDLC System
Length (Metric)15 mm
Max. Pressure7250 psi (500 bar)
Particle Size5 μm
pH2 to 8
Pore Size100 Å
Surface Area450 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load15%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
MaterialStainless Steel
TypeTrap Cartridge
Unit SizeEach
164197Full specifications
Pack of 25 μm0.1 mm10 mmTrap Column
305.00
Your Price:
***
Column FormatTrap Column
Diameter (Metric)0.1 mm
Length (Metric)10 mm
Max. Pressure7250 psi (500 bar)
Particle Size5 μm
pH2 to 8
Pore Size100 Å
Surface Area450 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load15%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
MaterialFused Silica
TypeTrap Column
Unit SizePack of 2
Showing 10 of 34Show more
  • High resolution in protein identification, biomarker discovery, and systems biology
  • Particular loadability allows highest sensitivity in LC/MS
  • Designed for TFA-free LC-MS, minimizing ion-suppression effects
  • Optimized for coupling to ESI/MS and MALDI-MS
  • Highest column-to-column reproducibility
  • Easy-to-use, cutting-edge miniaturized HPLC
  • Preassembled with nanoViper column inlet fittings for easy column installation

Key advantages of nanoViper fittings

  • Ease of use, no sleeve for small capillaries
  • Tool-free assembly
  • No column damage from overtightening: capable of withstanding pressures up to 1000 bars and is compatible with third-party valves and unions
  • No experimental failure due to bad connections

Ideal for high-resolution peptide mapping of tryptic, natural, and synthetic peptides as well as LC-MS/MS peptide mapping for protein identification, biomarker discovery, and systems biology.

  • High loading capacity
  • Use for the analysis of low abundant peptides often found in complex proteomics samples
  • Select the column length and I.D. for your throughput, sensitivity, and amount of sample available requirements
  • Choose from 50, 150, 250, 500mm lengths and 50, 75, 300 and 1000µm I.D.
  • Superior protein and peptide separations
  • Trifluoroacetic acid (TFA)-free LC/MS minimizes suppression effects for enhanced MS sensitivity
  • Outstanding separation efficiency
  • Superior resolution and recovery
  • Easy installation with nanoViper™ Fingertight Fittings

PepMap nanocolumns are also available pre-assembled with Thermo Scientific™ Dionex™ nanoViper™ Fingertight Fitting for a dead-volume-free connection to 1000 bar.

  • Requires no tools
  • Provides virtually zero-dead-volume connections
  • Withstands pressures up to a 1000 bar

About Nano, capillary, and micro Acclaim PepMap columns

Nano (75µm), capillary (300µm) and micro (1000µm) columns are ideal for protein and peptide analyses. Sample amount is often limited and smaller I.D. columns can increase in sensitivity in sample-limited situations. While nano LC columns are often used in proteomics, the concentrating effects of capillary and microcolumns are beneficial for metabolomics or for facilitating LC-MS interfacing. Thermo Scientific Acclaim PepMap columns are offered in nano, capillary, and microcolumn in a variety of chemistries for peptides and protein analysis.

Use PepMap Trap columns for desalting and preconcentration of peptides before LC separation with MS detection. The trap columns provide the highest efficiencies for one-dimensional peptide mapping experiments and 2D-LC analyses.

Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.

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Frequently asked questions (FAQs)

Citations & References