POROS® EP 20 µm Column, 4.6 x 50 mm, 0.8 mL

Catalog number: 1612224

Applied Biosystems™  Related applications: Chromatography | Protein Purification & Isolation

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Description

The POROS® Prepacked Immobilized Epoxide Affinity Column is based on an epoxide functional group designed for the immobilization of proteins through free amine groups. 20 micron particle size ensures high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS® Perfusion Chromatography® Media and Pre-Packed Columns
A high performance chromatography media for analytical to process scale separations.
• Higher productivity: High throughput and high dynamic capacity associated with Perfusion Chromatography®
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000Å
• Polystyrenedivinylbenzene particles yield a robust, easily packable matrix.
• Develop better separations methods in a shorter time frame: The speed of Perfusion Chromatography® technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation.

Reduce time-consuming sample prep
Replace many sample preparation steps with high speed Perfusion Chromatography® technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis
Perfusion Chromatography® technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High Capacity, High Resolution, High Speed
In contrast to conventional chromatography media, POROS® Perfusion Chromatography® media particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Column
Flow Rate: 1000 to 5000 cm⁄hr
Column Type: Analytical Column, Affinity Column
Product Size: 1 column
Particle Size: 20 micron
Support Matrix: Polystyrenedivinylbenzene
Immobilized Phase: Epoxide
Shipping Condition: Room Temperature
Sample Type (Specific): Protein
For Use With (Equipment): HPLC
High Throughput Compatibility: Not High Throughput-Compatible (Manual)

Contents & storage

Store at room temperature.

Documents

Manuals & protocols