The Thermo Scientific pMCS-Gaussia Luc vector is a multiple cloning site plasmid designed to accept a promoter sequence for study of gene regulation using the naturally secreting Gaussia luciferase reporter.
Features of the pMCS-Gaussia Luc vector:
• Naturally-secreting Gaussia luciferase gene, optimized for high expression in mammalian systems • Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another • Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through • Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively • High-copy pUC bacterial DNA replication origin
The Gaussia Luc Vectors contain a gene cloned from the copepod, Gaussia princeps. The gene encodes a naturally-secreted bioluminescent Gaussia luciferase (approx. 20kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates. The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.
These vectors are subject to a limited use label license.