Thermo Scientific™

pMCS-Gaussia Luc Vector for Luciferase Assays

Catalog number: 16146
Thermo Scientific™

pMCS-Gaussia Luc Vector for Luciferase Assays

Catalog number: 16146
Catalog Number
16146
Unit Size
10 µg
Price (USD)
Price: 105.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
1614610 µg
Price: 105.00
Your Price:
-
Product Overview
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The Thermo Scientific pMCS-Gaussia Luc vector is a multiple cloning site plasmid designed to accept a promoter sequence for study of gene regulation using the naturally secreting Gaussia luciferase reporter.

Features of the pMCS-Gaussia Luc vector:

• Naturally-secreting Gaussia luciferase gene, optimized for high expression in mammalian systems
• Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin

The Gaussia Luc Vectors contain a gene cloned from the copepod, Gaussia princeps. The gene encodes a naturally-secreted bioluminescent Gaussia luciferase (approx. 20kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates. The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.

These vectors are subject to a limited use label license.

More Product Data
Highly sensitive multiplex luciferase reporter assays
Luciferase assays in hard-to-transfect Jurkat cells
Monitoring neuronal differentiation using multiplexed luciferase reporters
Activation of the antioxidant response pathway by pesticide chemicals

Related Products
pCMV-Gaussia Luc Vector for Luciferase Assays
pTK-Gaussia Luc Vector for Luciferase Assays
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Description
pMCS-Gaussia Luc Vector
Type
Luc Vector
Cloning Method
Restriction Enzyme/MCS
Delivery Type
Transfection
Quantity
10 µg
Reporter Gene
Gaussia Luciferase
Selection Agent (Eukaryotic)
Puromycin
Product Line
Pierce™
For Use With (Application)
Reporter Assays

Contents & Storage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.