pCMV-Gaussia Luc Vector for Luciferase Assays

Catalog number: 16147

Thermo Scientific™  Related applications: Protein Assays and Analysis | Protein Expression

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Description

The Thermo Scientific pCMV-Gaussia Luc vector is a derivative of the pMCS-Gaussia Luc vector containing the Gaussia luciferase gene under the control of the CMV (Cytomegalovirus) promoter. This constitutive expression vector can be used as a normalization control to account for experimental variation in combination with other reporters.

Features of the pCMV-Gaussia Luc vector:

• Strong (CMV) constitutive promoters for co-transfection and normalization
• Naturally-secreting Gaussia luciferase gene, optimized for high expression in mammalian systems
• Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin

The Gaussia Luc Vectors contain a gene cloned from the copepod, Gaussia princeps. The gene encodes a naturally-secreted bioluminescent Gaussia luciferase (approx. 20kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

These vectors are subject to a limited use label license.

More Product Data
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Related Products
pMCS-Gaussia Luc Vector for Luciferase Assays
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For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV
Key Function: Reporter Assays
Reporter: Gaussia Luciferase
Delivery Method: Transfection
Cloning Method: Restriction Enzyme ⁄ MCS
Selection Agent (Eukaryotic): Puromycin
Product Size: 10 µg

Contents & storage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.

Documents

Manuals & protocols