pTK-Cypridina Luc Vector for Luciferase Assays

Catalog number: 16151

Thermo Scientific™  Related applications: Protein Assays and Analysis | Protein Expression

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Description

The Thermo Scientific pTK-Cypridina Luc Vector is a multiple cloning site plasmid designed to accept a promoter sequence for study of gene regulation using the naturally secreting Cypridina luciferase reporter.

The Cypridina Luc Vectors contain a gene cloned from the marine ostracod, Cypridina noctiluca. The gene encodes a naturally-secreted bioluminescent Cypridina luciferase (62kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates. The pTK vector has the luciferase gene under the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, and this constitutive expression vector can be used as a normalization control to account for experimental variation in combination with other reporters.

Features of the Cypridina Luc Vectors:

• Naturally-secreting Cypridina luciferase gene, optimized for high expression in mammalian systems
• Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin
• Two control vectors available with strong (CMV) or weak (TK) constitutive promoters for co-transfection and normalization

The pMCS-, pCMV- and pTK-Cypridina Luc Vectors encode the Cypridina luciferase reporter with excellent light intensity.

These vectors are subject to a limited use label license.

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Related Products
pMCS-Cypridina Luc Vector for Luciferase Assays
pCMV-Cypridina Luc Vector for Luciferase Assays
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: TK
Key Function: Reporter Assays
Reporter: Cypridina Luciferase
Delivery Method: Transfection
Cloning Method: Restriction Enzyme ⁄ MCS
Selection Agent (Eukaryotic): Puromycin
Product Size: 10 µg

Contents & storage

Store between -70°C and -20°C.

Documents

Manuals & protocols