pMCS-Red Firefly Luc Vector for Luciferase Assays

Catalog number: 16155

Thermo Scientific™  Related applications: Protein Assays and Analysis | Protein Expression

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Thermo Scientific pMCS-Red Firefly Luc is a multiple cloning site plasmid designed to accept a promoter sequence for the study of gene regulation using the intracellular red firefly luciferase reporter with excellent light intensity..

Features of pMCS-Red Firefly Luc:

• Intracellular Red Firefly Luciferase gene, optimized for high expression in mammalian systems
• Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin
• Two control vectors available with strong (CMV) or weak (TK) constitutive promoters for co-transfection and normalization

Features of Red Firefly Luc:
• Red Firefly Luc Vectors contains a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum.
• The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.
• The pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively.
• These pCMV and pTK constitutive expression vectors can be used as normalization controls to account for experimental variation in combination with other reporters.

These vectors are subject to a limited use label license.

More Product Data
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Related Products
pCMV-Red Firefly Luc Vector for Luciferase Assays
pTK-Red Firefly Luc Vector for Luciferase Assays
For Research Use Only. Not for use in diagnostic procedures.


Key Function: Reporter Assays
Reporter: Red Firefly Luciferase
Delivery Method: Transfection
Cloning Method: Restriction Enzyme ⁄ MCS
Selection Agent (Eukaryotic): Puromycin
Product Size: 10 µg

Contents & storage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.


Manuals & protocols

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