Thermo Scientific™

pTK-Gaussia-Dura Luc Vector for Luciferase Assays

Catalog number: 16192
Thermo Scientific™

pTK-Gaussia-Dura Luc Vector for Luciferase Assays

Catalog number: 16192
Catalog Number
16192
Unit Size
10 µg
Price (USD)
Price: 106.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
1619210 µg
Price: 106.00
Your Price:
-
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The Thermo Scientific pTK-Gaussia-Dura Luc vector is a derivative of pMCS-Gaussia-Dura Luc. It contains a mutant form of the Gaussia luciferase gene (conferring better bioluminescent signal stability than the native luciferase) under the control of the weak Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter. This constitutive expression vector can be used as a normalization control to account for experimental variation in combination with other reporters.

Features of the pTK-Gaussia-Dura Luc vector:

• Weak (TK) constitutive promoter for co-transfection and normalization
• Naturally-secreting Gaussia-Dura luciferase gene, optimized for high expression and glow-stability in mammalian systems
• Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin

Gaussia-Dura luciferase (approx. 20kDa) is a secreted protein that enables measurement of the reporter activity in media (for real-time assays) and in cell lysates. The parent pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.

These vectors are subject to a limited use label license.

Related Products
pMCS-Gaussia-Dura Luc Vector for Luciferase Assays
pCMV-Gaussia-Dura Luc Vector for Luciferase Assays
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Description
pTK-Gaussia-Dura Luc Vector
Type
Luc Vector
Cloning Method
Restriction Enzyme/MCS
Delivery Type
Transfection
Quantity
10 µg
Reporter Gene
Gaussia Dura Luciferase
Selection Agent (Eukaryotic)
Puromycin
For Use With (Application)
Reporter Assays
Promoter
TK

Contents & Storage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.