POROS® OH 20 µm Self Pack® Media

Catalog number: 1632810

Applied Biosystems™  Related applications: Chromatography | Protein Purification & Isolation

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Description

Applied Biosystems® POROS® Activated Affinity Media. Contains enough packing for three 4.6 mm x 100 mm columns or six 4.6 mm x 50 mm columns. POROS® OH is based on an hydroxylated functional group designed for the immobilization of proteins through activation with tresyl-chloride, divinyl-sulphone or cyanogen bromide.
20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS® Self Pack® Column Packing Technology

Self Pack Technology allows you to pack your own high performance POROS columns at a fraction of the cost of prepacked columns. As such, it represents the most cost-effective way to screen the range of POROS media for the one best suited for the separation problem at hand. The Self Pack Technology consists of four elements (each sold separately): a packing device, an empty column, POROS media and an appropriate test standard.

POROS® Perfusion Chromatography® Media and Pre-Packed Columns

A high performance chromatography media for analytical to process scale separations.
• Higher productivity: High throughput and high dynamic capacity associated with Perfusion Chromatography®
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of Perfusion Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed Perfusion Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

Perfusion Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS® Perfusion Chromatography® media particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Column Type: Affinity Column
Format: Self Pack Media
Immobilized Phase: Hydroxyl
Product Size: 3 g
Shipping Condition: Room Temperature

Contents & storage

Store at room temperature.

Documents

Manuals & protocols