Can I use an end-labeled probe with the North2South Chemiluminescent Hybridization and Detection Kit?
Short probes that are end-labeled with biotin may work with optimization to improve sensitivity. For longer probes, however, random-prime labeled produces better results because it adds multiple biotin group per molecule of probe.
How can I biotinylate my probe for the North2South Chemiluminescent Hybridization and Detection Kit?
Biotinylated DNA probes can be generated with the North2South Biotin Random Prime DNA Labeling Kit (Cat. No. 17075). Probes may also be generated by PCR, random primed labeling or in vitro transcription. Biotinylated probes made with reagents and kits from other companies are compatible.