Isolation, cultivation and characterization of adult murine prostate stem cells.
AuthorsLukacs RU, Goldstein AS, Lawson DA, Cheng D, Witte ON,
JournalNat Protoc
PubMed ID20360765
'The successful isolation and cultivation of prostate stem cells will allow us to study their unique biological properties and their application in therapeutic approaches. Here we describe step-by-step procedures on the basis of previous work in our laboratory for the harvesting of primary prostate cells from adolescent male mice by ... More
Production of hepatocyte-like cells from human pluripotent stem cells.
Large-scale production of hepatocytes from a variety of genetic backgrounds would be beneficial for drug screening and to provide a source of cells to be used as a substitute for liver transplantation. However, fully functional primary hepatocytes remain difficult to expand in vitro, and circumventing this problem by using an ... More