Can I use a Nunc Cell Scraper with a Nunc TripleFlask that has Nunclon Delta surface treatment?
The design of the Nunc TripleFlask does not allow for mechanical scraping. However, cells are easily harvested using protein digesting enzymes, such as trypsin, collagenase, and Pronase or by using non-enzymatic dissociation solutions.
The following is a document link regarding culturing techniques for Nunc TripleFlask:
https://assets.thermofisher.com/TFS-Assets/BID/Technical-Notes/culturing-technique-nunc-tripleflask-technical-note.pdf
The following is a document link regarding culturing techniques for Nunc TripleFlask:
https://assets.thermofisher.com/TFS-Assets/BID/Technical-Notes/culturing-technique-nunc-tripleflask-technical-note.pdf