Here, we describe optimal conditions for the culture of rainbow trout (Oncorhynchus mykiss) pre-adipocytes obtained from adipose tissue and their differentiation into mature adipocytes, in order to study the endocrine control of adipogenesis. Pre-adipocytes were isolated by collagenase digestion and cultured on laminin or 1% gelatin substrate. The expression of ... More
Effect of immunohistochemistry on molecular analysis of tissue samples: implications for microdissection technologies.
AuthorsTangrea MA, Mukherjee S, Gao B, Markey SP, Du Q, Armani M, Kreitman MS, Rosenberg AM, Wallis BS, Eberle FC, Duncan FC, Hanson JC, Chuaqui RF, Rodriguez-Canales J, Emmert-Buck MR
JournalJ Histochem Cytochem
PubMed ID21430260
'Laser-based tissue microdissection is an important tool for the molecular evaluation of histological sections. The technology has continued to advance since its initial commercialization in the 1990s, with improvements in many aspects of the process. More recent developments are tailored toward an automated, operator-independent mode that relies on antibodies as ... More
Diminished callus size and cartilage synthesis in alpha 1 beta 1 integrin-deficient mice during bone fracture healing.
AuthorsEkholm E, Hankenson KD, Uusitalo H, Hiltunen A, Gardner H, Heino J, Penttinen R,
JournalAm J Pathol
PubMed ID12000729
'Integrins mediate cell adhesion to extracellular matrix components. Integrin alpha 1 beta 1 is a collagen receptor expressed on many mesenchymal cells, but mice deficient in alpha 1 integrin (alpha1-KO) have no gross structural defects. Here, the regeneration of a fractured long bone was studied in alpha1-KO mice. These mice ... More
14-3-3s controls corneal epithelium homeostasis and wound healing.
AuthorsLu Q, Xin Y, Ye F, Foulks G, Li Q
JournalInvest Ophthalmol Vis Sci
PubMed ID21228373
'To investigate the functional role of 14-3-3s in regulation of the corneal epithelial proliferation, differentiation, and wound-healing response.'
Shenling Baizhu San supresses colitis associated colorectal cancer through inhibition of epithelial-mesenchymal transition and myeloid-derived suppressor infiltration.
AuthorsLin X, Xu W, Shao M, Fan Q, Wen G, Li C, Jing L, Sun X
Journal
PubMed ID25897964
Shenling Baizhu San (SBS) is a well-known and classical Chinese medicine formula. It has been used for treatment of gastrointestinal disorders for about nine hundred years. Recent reports showed that it was effective in curing colitis and ameliorating the major manifestations of postoperational colorectal cancer (CRC). This study was to ... More
Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1).
AuthorsSolter D, Knowles BB,
JournalProc Natl Acad Sci U S A
PubMed ID281705
A monoclonal antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with F9 teratocarcinoma cells is described. This antibody, which reacts with embryonal carcinoma cells of mouse and human origin and with some preimplantation stage mouse embryos, defines an embryonic stage-specific antigen. This stage-specific ... More
CDK14 expression is down-regulated by cigarette smoke in vivo and in vitro.
AuthorsPollack D, Xiao Y, Shrivasatava V, Levy A, Andrusier M, D'Armiento J, Holz MK, Vigodner M
Journal
PubMed ID25680692
In this study, DNA arrays have been employed to monitor gene expression patterns in testis of mice exposed to tobacco smoke for 24 weeks and compared to control animals. The results of the analysis revealed significant changes in expression of several genes that may have a role in spermatogenesis. Cdk14 ... More
Evaluation of prognostic factors in male breast cancer.
AuthorsTemmim L, Luqmani YA, Jarallah M, Juma I, Mathew M
JournalBreast
PubMed ID14965580
We conducted an analysis on 41 cases of male breast cancer (median age 54 y; range 25-82 y) in Kuwait. Most (51%) were stage II cancers with 65% arising in the left breast. There were 5 (12%) T1 tumours, 23 (56%) T2 tumours and 13 (32%) T3/T4 tumours. They were ... More
Developmental origins of species differences in telencephalon and tectum size: morphometric comparisons between a parakeet (Melopsittacus undulatus) and a quail (Colinus virgianus).
AuthorsStriedter GF, Charvet CJ
JournalJ Comp Neurol
PubMed ID18241052
Parrots, including parakeets, evolved significantly larger brains than other birds, relative to their body size, and they possess a proportionately larger telencephalon. For example, the telencephalon occupies approximately 68% of the brain in parakeets but only 52% in bobwhite quail. The present study was designed to determine when and how ... More